有无睑板腺功能障碍患者眼表菌群组成对比  

Comparison of ocular surface microbial colonies between patients with and without meibomian gland dysfunction

作　　者：曹宇辰 张杰鑫 黎颖莉 易果果 符敏[5] CAO Yuchen;ZHANG Jiexin;LI Yingli;YI Guoguo;FU Min(The Second Clinical School of Southern Medical University,Guangzhou 510280,Guangdong Province,China;The First Clinical School of Southern Medical University,Guangzhou 510515,Guangdong Province,China;Department of Ophthalmology,Shenzhen Hospital,Southern Medical University,Shenzhen 518000,Guangdong Province,China;Department of Ophthalmology,The Sixth Affiliated Hospital of Sun Yat-sen University,Guangzhou 510655,Guangdong Province,China;Department of Ophthalmology,Zhujiang Hospital,Southern Medical University,Guangzhou 510280,Guangdong Province,China)

机构地区：[1]南方医科大学第二临床医学院,广东省广州市510280 [2]南方医科大学第一临床医学院,广东省广州市510515 [3]南方医科大学深圳医院眼科,广东省深圳市518000 [4]中山大学附属第六医院眼科,广东省广州市510655 [5]南方医科大学珠江医院眼科,广东省广州市510280

出　　处：《眼科新进展》2021年第12期1138-1143,共6页Recent Advances in Ophthalmology

基　　金：国家自然科学基金(编号81800804);广东省自然科学基金(编号2017A030310108);广东省教育厅高水平大学建设资助南方医科大学临床研究启动项目(编号LC2016YM017)。

摘　　要：目的研究睑板腺功能障碍(MGD)与非MGD(NMGD)患者的眼表菌群的组成及差异。方法招募了57例MGD患者为MGD组,32名健康志愿者为NMGD组,对两组受试者结膜拭子进行16S rRNA基因V3-V4片段的高通量测序,对测序数据进行生物信息学分析,以研究MGD组患者和NMGD组受试者眼表菌群的组成及差异。结果MGD组患者和NMGD组受试者眼表菌群的16S rRNA的测序结果显示,眼表菌群多样性没有差异(均为P>0.05)。生物信息学分析结果显示,在门和属水平上两组受试者显示了相似的眼表菌群构成。MGD组患者中,I级、II级和III级患者Shannon指数(P=0.006)和Dominance指数(P=0.012)均存在显著差异。此外,使用未加权和加权的Unifrac方法构建的主坐标分析图也表明,I级、II级、III级MGD患者之间的样本区分明显(P=0.039)。结论有无MGD的患者眼表菌群没有显著差异。不同严重程度的MGD患者的眼表菌群存在显著差异。Objective To investigate the composition and difference of ocular surface(OS)microbial colonies between patients with meibomian gland dysfunction(MGD)and non-meibomian gland dysfunction(NMGD).Methods A total of 57 MGD patients were recruited in the MGD group,and 32 healthy volunteers were recruited in the NMGD group.The high-throughput sequencing of 16S rRNA V3-V4 fragments was performed using their conjunctival swab samples.The sequencing data was analyzed bioinformatically to explore the composition of the OS microbial colonies in MGD and NMGD patients and their difference.Results The 16S rRNA sequencing data showed that there was no significant difference in the diversity of OS microbial colonies between the MGD group and the NMGD group(both P>0.05).The bioinformatic analysis results showed there was a similar OS microbial structure at the phylum and genus levels between the two groups.In the MGD group,Grade I,Grade II and Grade III MGD patients showed significant differences in Shannon’s index(P=0.006)and dominance index(P=0.012).Furthermore,the principal coordinate analysis chart constructed using the unweighted and weighted Unifrac method also demonstrated a significant difference among samples of patients with Grade I,Grade II and Grade III MGD(P=0.039).Conclusion There is no significant difference in the OS microbial colonies between MGD patients and NMGD patients.However,there is a significant difference in the OS microbial colonies among patients with different grades of MGD.

关 键 词：睑板腺功能障碍 眼表菌群 16S rRNA高通量测序 

分 类 号：R777.1[医药卫生—眼科]