纳米硫化铜复合物光热治疗对舌鳞状细胞癌的体内外抑制作用  

Photothermal effect of nano-copper sulfide against tongue squamous cell carcinoma

作　　者：陈栋[1] 陈之锋[1] 王邹 杨雅洁[1] 蒋艳萍 胡琛[1] CHEN Dong;CHEN Zhifeng;WANG Zou;YANG Yajie;JIANG Yanping;HU Chen(Department of Stomatology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China)

机构地区：[1]南方医科大学南方医院口腔科,广东广州510515

出　　处：《南方医科大学学报》2021年第12期1843-1849,共7页Journal of Southern Medical University

基　　金：国家自然科学基金青年基金(82001298)。

摘　　要：目的探讨BSA@CuS-PEG纳米复合物的制备、表征、功能及其对舌癌的抗肿瘤效应的作用。方法采用透射电镜,动态光散射,Zeta电位分析及紫外吸收光谱分析方法分析BSA@CuS-PEG纳米复合物的表征,利用近红外II区激发光(1064 nm)探究其光热性能,同时采用CCK-8实验评估该纳米复合物的细胞毒性,流式细胞学实验分析细胞周期分布水平。最后,构建单侧皮下荷瘤模型探究BSA@CuS-PEG纳米复合物的体内抗肿瘤作用。结果成功制备BSA@CuS-PEG纳米复合物,在0.5 W/cm^(2)功率的近红外光照射下其变化温度值(ΔT)约为30℃,具有优良的光热性能。CCK8实验表明:单纯BSA@CuS-PEG对肿瘤细胞无明显毒性;而相比于纯材料组,BSA@CuS-PEG联合近红外光照射对Cal27及SCC9舌癌细胞具有显著抑制增殖作用(P<0.001)。同时细胞周期分析显示:相比于纯材料组,BSA@CuS-PEG联合近红外光照射使Cal27及SCC9舌癌细胞更多被阻滞于G2/M期(P<0.001)。小鼠单侧皮下荷瘤模型表明:相比于生理盐水联合近红外光照射处理组,BSA@CuS-PEG联合近红外光照射发挥显著的体内抗肿瘤作用(P<0.001)。结论BSA@CuS-PEG纳米复合物具有良好的光热效应,体内外抗肿瘤效应,为舌癌原发灶无创早期诊断和非手术治疗提供一种潜在思路和手段。Objective To investigate the anti-tumor effect of BSA@CuS-PEG nanocomposites on tongue squamous cell carcinoma.Methods Transmission electron microscopy,dynamic light scattering,Zeta potential and ultraviolet absorption spectroscopy were used to characterize the synthesized BSA@CuS-PEG nanocomposite,whose photothermal properties was assessed with near infrared II region excitation light(1064 nm).The cytotoxicity of the nanocomposite in Cal27 and SCC9 cells was evaluated using CCK-8 assay,and its effect on cell cycle distribution was analyzed using flow cytometry.The in vivo antitumor effect of BSA@CuS-PEG was investigated in a Balb/c mouse model bearing subcutaneous Cal27 tumor xenograft.Results The synthesized BSA@CuS-PEG nanocomposite showed a temperature variation(ΔT)of about 30℃ under near-infrared(NIR)irradiation(0.5 W/cm^(2)),suggesting its excellent photothermal sensitivity.CCK-8 assay showed that BSA@CuS-PEG had no significant toxicity to tumor cells,but upon NIR irradiation,the nanocomposite produced a significant stronger inhibitory effect on Cal27 and SCC9 cells than free nanocomposites(P<0.001).Cell cycle analysis showed that compared with free nanocomposites,BSA@CuS-PEG plus NIR irradiation caused more obvious cell cycle arrest at G2/M phase in tongue cancer cells(P<0.001).In the tumor-bearing mice,BSA@CuS-PEG combined with NIR irradiation produced a significant anti-tumor effect as compared with saline treatment plus NIR irradiation(P<0.001).Conclusion The BSA@CuS-PEG nanocomposite shows prominent photothermal properties and good anti-tumor effects both in vivo and in vitro,and thus provides a promising method for non-invasive early diagnosis and non-surgical treatment of primary tongue cancer.

关 键 词：光热治疗 硫化铜 舌癌 纳米复合物 

分 类 号：R739.8[医药卫生—肿瘤]