红景天苷衍生物对苯甲酰红景天苷对MCAO大鼠的神经保护作用  

The neuroprotective effect of p-benzoyl salidroside, a derivative of salidroside, on MCAO model rats

作　　者：聂婧雯 孙春晓 黄私迎 罗锐 赖文芳[1] 王颖峥 洪桂祝 NIE Jing-wen;SUN Chun-xiao;HUANG Si-ying;LUO Rui;LAI Wen-fang;WANG Ying-zheng;HONG Gui-zhu(College of Pharmacy,Fujian University of Tranditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学药学院,福建福州350122

出　　处：《中国药理学通报》2022年第1期67-72,共6页Chinese Pharmacological Bulletin

基　　金：福建省卫生健康科研人才培养青年人才项目(No 2019-1-64)。

摘　　要：目的研究红景天苷衍生物对苯甲酰红景天苷(p-benzoyl salidroside, pOBz)对大脑中动脉闭塞模型(MCAO)大鼠的神经保护作用。方法 (1)30只健康成年♂SD大鼠,随机分为Sham组、MCAO组、MCAO+pOBz低中高剂量组(25、50、100 mg·kg^(-1)),线栓法造模,进行神经功能损伤评分,连续给药2 d。采用核磁共振成像检测MCAO大鼠脑梗死体积。(2)24只健康成年♂SD大鼠,随机分为Sham组、MCAO组、MCAO+pOBz组(50 mg·kg^(-1))和MCAO+Sal组(50 mg·kg^(-1)),线栓法造模,给药1 d。Western blot检测NeuN、EGR1、Bcl-2和Bax蛋白表达。(3)18只健康成年♂SD大鼠,随机分成Sham组、MCAO组和MCAO+pOBz组(50 mg·kg^(-1)),连续给药2 d。免疫荧光染色观察NeuN的表达。结果 pOBz给药2 d可以降低MCAO大鼠的脑梗死体积,改善神经功能损伤,且升高NeuN和EGR1表达程度优于Sal, pOBz对MCAO大鼠脑组织Bcl-2/Bax比值的改善程度与Sal相当。结论 pOBz可改善MCAO大鼠脑梗死体积,具有优于红景天苷的神经保护作用。Aim To study the neuroprotective effect of p-benzoyl salidroside(pOBz), a derivative of salidroside, on MCAO model rats. Methods(1) Thirty healthy adult male SD rats were randomly divided into Sham group, MCAO group and MCAO+pOBz group(25, 50, 100 mg·kg^(-1)). MCAO model was made by suture-embolus method. The rats were scored for neurological function impairment and weighed every day. pOBz was intraperitoneally injected and administered continuously for two days after preparation of MCAO model. The cerebral infarction volume of rats was detected by MRI.(2) Twenty-four healthy adult male SD rats were randomly divided into Sham group, MCAO group, MCAO+pOBz group(50 mg·kg^(-1)) and MCAO+Sal group(50 mg·kg^(-1)). The model was made by the suture-embolus method. pOBz was intraperitoneally injected and administered continuously for one day. Western blot was used to detect the expression of NeuN, EGR1, Bcl-2 and Bax.(3) Eighteen healthy adult male SD rats were randomly divided into Sham group, MCAO group and MCAO+pOBz group(50 mg·kg^(-1)). Administration continued for 2 days. Immunofluorescence staining was used to detect the expression of NeuN. Results Intraperitoneal injection of pOBz for 2 days could reduce the cerebral infarction volume of MCAO rats, improve neurological impairment and increase the expression of NeuN and EGR1, and the effect was better than that of Sal. pOBz improved Bcl-2/Bax in brain tissues of MCAO rats to the same extent as Sal did. Conclusions pOBz can reduce the volume of cerebral infarction in MCAO rats and has better neuroprotective effect than that of salidroside.

关 键 词：对苯甲酰红景天苷 缺血性脑卒中 MCAO 神经保护 NEUN EGR1 

分 类 号：R-332[医药卫生] R282.71