我国北方香型粳稻资源遗传多样性及其香味基因Badh2的等位基因分析  

Analyses of Genetic Diversity and Badh2 Alleles of Aromatic Japonica Rice Germplasm Resources in Northern China

作　　者：朴日花[1] 金永梅[2] 李萍 陈莫军[1] 孟凡梅[1] 周广春[1] 严永峰[1] PIAO Rihua;JIN Yongmei;LI Ping;CHEN Mojun;MENG Fanmei;ZHOU Guangchun;YAN Yongfeng(Rice Research Institute,Jilin Academy of Agricultural Sciences,Gongzhuling 136100,China;Agro-Biotechnology Research Institute,Jilin Academy of Agricultural Sciences,Changchun 130033,China;College of Basic Science,Tianjin Agricultural University,Tianjin 300384,China;International Joint Research Center of Technology Innovation and Achievement Transformation on Palatability of Rice,Tianjin Agricultural University,Tianjin 300384,China)

机构地区：[1]吉林省农业科学院水稻研究所,公主岭136100 [2]吉林省农业科学院农业生物技术研究所,长春130033 [3]天津农学院基础科学学院,天津300384 [4]天津农学院天津市食味水稻科技创新与成果转化国际联合研究中心,天津300384

出　　处：《吉林农业大学学报》2021年第5期507-515,共9页Journal of Jilin Agricultural University

基　　金：吉林省农业科技创新工程项目(CXGC2019DC003,CXGC2020DX004);国家现代农业产业技术体系项目(CARS-01-10)。

摘　　要：为了解我国北方香型粳稻资源遗传背景,利用SNP分子标记对54份香型粳稻和8份非香稻资源进行遗传多样性分析,并对香味基因Badh2进行等位基因分析。1K SNP分子标记基因分型结果共检测出2 244个等位位点,62份材料的相似系数为0.18~0.99,平均系数为0.81。聚类分析结果表明:62份材料共分为3个类群(相似系数为0.82),然而类群并未按不同地域完全分开,其中只有第一类群材料大多来自吉林省。利用已开发的香味关联分子标记,鉴定54份香稻资源的Badh2基因等位变异类型,发现2份资源具有新等位变异,大部分材料为第7外显子和第2外显子变异类型。以上结果表明,我国北方香型粳稻资源的遗传距离较近、遗传多样性较低,这可为北方香型粳稻资源研究及香稻育种亲本选配提供参考依据。In order to understand the genetic background of aromatic japonica rice resources in northern China,the genetic diversity of 54 aromatic-and 8 non-aromatic japonica rice resources was analyzed by using SNP molecular markers and the allele analysis of fragrant gene Badh2.The results of 1 K SNP genotyping showed that a total of 2,244 alleles were detected in 62 materials,and the similarity coefficient ranged from 0.18 to 0.99 with the mean coefficient of 0.81.The cluster analysis of Nei′s similarity coefficient showed that the 62 varieties were divided into 3 groups(similarity coefficient was 0.82),but not completely separated according to regions,and only the materials in the first group were mostly from Jilin province.Allele analysis of fragrant gene Badh2 using the developed aroma-associated molecular markers showed that most aromatic rice resources were Exon 7 and Exon 2 mutation types,and 2 of them were the novel alleles.The results indicated that the genetic distance and genetic diversity of aromatic japonica rice resources in northern China were relatively narrow.This study provides a theoretical basis for the study of aromatic type japonica rice resources and breeding in northern China.

关 键 词：香稻 粳稻 遗传多样性 Badh2 等位基因 

分 类 号：S511[农业科学—作物学]