CircRNA_23113抑制肺腺癌细胞的增殖和迁移  

CircRNA_23113 inhibits the proliferation and migration of lung adenocarcinoma cells

作　　者：王灵霞 严玉兰[1] 袁海涛 蔡烨伟 刘德慧 谷文露 曹碧月 WANG Ling-xia;YAN Yu-lan;YUAN Hai-tao;CAI Ye-wei;LIU De-hui;GU Wen-lu;CAO Bi-yue(Department of Respiratory Medicine,the Affiliated People's Hospital of Jiangsu University,Zhenjiang 212002;Department of General Surgery, the Affiliated People's Hospital of Jiangsu University,Zhenjiang 212002;Department of Respiratory Medicine, Shanghai Songjiang District Central Hospital, Shanghai 201600, China)

机构地区：[1]江苏大学附属人民医院呼吸内科,江苏镇江212002 [2]江苏大学附属人民医院普外科,江苏镇江212002 [3]上海市松江区中心医院呼吸内科,上海201600

出　　处：《基础医学与临床》2022年第1期82-88,共7页Basic and Clinical Medicine

基　　金：江苏省自然科学基金(BK20151333);镇江市重点研发计划(SH2018059)。

摘　　要：目的探究circRNA_23113在肺腺癌中的表达及对肺腺癌细胞增殖和迁移的影响。方法通过高通量测序技术测序5例临床肺腺癌、癌旁组织标本,筛选出差异性表达的circRNA_23113。实时荧光定量PCR检测肺腺癌组织、血清和细胞中circRNA_23113的表达;构建circRNA_23113的过表达质粒,用CCK-8法、克隆形成实验、细胞划痕和Transwell小室法分析其对细胞增殖和迁移的影响;用Western blot检测β-catenin、cyclin D1以及c-myc蛋白水平的表达。结果CircRNA_23113在肺腺癌患者组织、血清和细胞中显著低表达(P<0.05);过表达circRNA_23113后抑制A549和H1299细胞增殖和迁移(P<0.05);CircRNA_23113抑制β-catenin、cyclin D1以及c-myc表达(P<0.05)。结论CircRNA_23113在肺腺癌中低表达,且过表达能抑制肺腺癌细胞的增殖和迁移。Objective To explore the expression of circRNA_23113 in lung adenocarcinoma and its influence on the proliferation/migration of the adenocarcinoma cells.Methods Screening 5 cases of lung adenocarcinoma and adjacent tissues by high-throughput sequencing from which screened out the differentially expressed circular RNA circRNA_23113.Real-time fluorescence quantitative PCR was used to detect the expression of circRNA_23113 in the adenocarcinoma tissues,serum and cells;the over-expression plasmid of circRNA_23113 was constructed,and its effect on cell proliferation and migration was measured by CCK-8 method,clone formation experiment,cell scratching and Transwell chamber method;Western blot was used to detect the expression ofβ-catenin,cyclin D1 and c-myc protein.Results CircRNA_23113 was significantly less expressed in tissues,serum and cells of patients with lung cancer(P<0.05);Over-expression of circRNA_23113 inhibited the proliferation and migration of A549 and H1299 cells(P<0.05);CircRNA_23113 inhibited the expression ofβ-catenin,cyclin D1 and c-myc(P<0.05).Conclusions CircRNA_23113 is poorly expressed in lung adenocarcinoma,and over-expression may inhibit the proliferation and migration of lung adenocarcinoma cells.

关 键 词：circRNA_23113 肺腺癌 增殖 迁移 

分 类 号：R734.2[医药卫生—肿瘤]