金黄色葡萄球菌确证试验的探讨  

Study on the identification test for Staphylococcus aureus

作　　者：张菁 ZHANG Jing(Taizihe District Health Supervision Center of Liaoyang City,Liaoyang,Liaoning 111000,China)

机构地区：[1]辽阳市太子河区卫生健康监督中心,辽宁辽阳111000

出　　处：《中国国境卫生检疫杂志》2021年第6期388-390,共3页Chinese Journal of Frontier Health and Quarantine

摘　　要：目的比较3种生化试验对金黄色葡萄球菌的鉴定结果,探索更适用于金黄色葡萄球菌的鉴定方法。方法以2011—2020年从食品样本中分离的138株葡萄球菌为研究对象,其中金黄色葡萄球菌23株,非金黄色葡萄球菌115株,对其进行血浆凝固酶试验、甘露醇试验及肠毒素检测,比较对金黄色葡萄球菌鉴定的灵敏度、特异度、阳性预测值、阴性预测值和粗一致性。结果血浆凝固酶试验对金黄色葡萄球菌鉴定的灵敏度为100.00%,特异度为94.78%,阳性预测值为79.31%,阴性预测值为100.00%,粗一致性为95.65%;肠毒素试验对金黄色葡萄球菌鉴定的灵敏度、特异度、阳性预测值、阴性预测值和粗一致性均为100.00%;甘露醇试验对金黄色葡萄球菌鉴定的灵敏度为100.00%,特异度为80.87%,阳性预测值为51.11%,阴性预测值为100.00%,粗一致性为84.06%。结论肠毒素试验对金黄色葡萄球菌鉴定的特异度、阳性预测值和粗一致性高于血浆凝固酶试验,血浆凝固酶试验又高于甘露醇试验。肠毒素试验与金黄色葡萄球菌鉴定有更好的相关性,更适合作为金黄色葡萄球菌鉴定的确证方法。Objective To explore a more suitable method for the determination of Staphylococcus aureus by comparing three identification tests.Methods A total of 138 strains of Staphylococcus isolated from food samples were collected during 2011-2020 as the research objects,in which of them,23 strains were Staphylococcus aureus and115 strains were other Staphylococcus.Plasma coagulase test,mannitol test and enterotoxin test were performed to all the strains.The sensitivity,specificity,positive predictive value,negative predictive value and coarse consistency for Staphylococcus aureus identification were calculated.Results The sensitivity,specificity,positive predictive value,negative predictive value and crude consistency of plasma coagulase test for Staphylococcus aureus was 100.00%,94.78%,79.31%,100.00% and 95.65%,respectively.The sensitivity,specificity,positive predictive value,negative predictive value and crude consistency of enterotoxin test for Staphylococcus aureus identification were all 100.00%.The sensitivity,specificity,positive predictive value and negative predictive value of mannitol test for Staphylococcus aureus identification was 100.00%,80.87%,51.11%,100.00% and 84.06%,respectively.Conclusion The specificity,positive predictive value and crude consistency of the enterotoxin test for Staphylococcus aureus identification were significantly higher than that of the plasma coagulase test,which was also significantly higher than that of the mannitol test.Enterotoxin test showed better correlation with the identification of Staphylococcus aureus,which is more suitable for the identification of Staphylococcus aureus.

关 键 词：金黄色葡萄球菌 肠毒素试验 血浆凝固酶试验 食源性细菌 

分 类 号：R155.31[医药卫生—营养与食品卫生学]