植株叶绿素a和叶绿素b无损检测系统研究与开发  

Plant Chlorophyll a and Chlorophyll b Nondestructive Testing System:Research and Development

作　　者：杨张青 李国强 张玉民 苏伟波 吴建中 段铁城 齐磊[2] Yang Zhangqing;Li Guoqiang;Zhang Yumin;Su Weibo;Wu Jianzhong;Duan Tiecheng;Qi Lei(Institute of Agricultural Economics and Information,Henan Academy of Agricultural Sciences,Zhengzhou 450008;Henan Nongda Xunjie Measurement and Testing Technology Co.,Ltd.,Zhengzhou 450002;Henan Agricultural University,Zhengzhou 450002)

机构地区：[1]河南省农业科学院农业经济与信息研究所,郑州450008 [2]河南农大迅捷测试技术有限公司,郑州450002 [3]河南农业大学,郑州450002

出　　处：《中国农学通报》2021年第34期147-152,共6页Chinese Agricultural Science Bulletin

基　　金：河南省重点研发与推广专项(科技攻关)“河南省农作物生产技术信息服务系统研究”(212102110255);农产品农残实时检测监控云平台关键技术研发(192102110048);国家重大专项“土壤面源污染和重金属检测设备的研制”(2017YFD0801200)。

摘　　要：针对透射式叶绿素检测技术在检测较高含量叶绿素叶片时的局限性,研究设计基于光学漫反射式新颖的叶绿素检测系统,实现无损在线测试,直接输出植株叶片叶绿素a和b值。该检测系统采用3光源3光束垂直入射密闭光路设计,选用645 nm和663 nm 2个特征波长作为叶绿素a和b的检测波长,800 nm作为参照波长;采用光源调制、高通滤波放大和软件算法等消除外界光干扰;以透光度75%的消光片对该检测系统进行稳定性和重复性测试评价。测评结果为稳定性RSD≤0.39%(n=20),重复性RSD≤0.59%(n=20)。选取小麦、玉米、花卉共344个样本,漫反射法和常规Arnon法的叶绿素a和b总量的相关性达到极显著水平;且适用于厚度约3.0 mm玻璃翠花叶片的检测。Aiming at the limitations of the transmission spectra for the detection of chlorophyll content at a high concentration,a novel optical detection system using the theory of diffuse reflection was developed.The detection system could show the concentration of chlorophyll a and b in plant leaves in real-time by nondestructive diagnostic technique.In this detection system,the light system was in an enclosed dark room,which adopted the design method of three light sources and three beams incident vertically,the characteristic wavelength of 645 nm and 663 nm were selected as the detection wavelength of Chlorophyll a and b,and the wavelength of 800 nm was the reference wavelength.The light source modulation,high-pass filter amplification and mathematical algorithm were used to eliminate the interference from the external lightsource.The stability and repeatability of the detection system were carefully evaluated with a 75%permeable extinction film.The stability with relative standard deviation(RSD)≤0.39%(n=20)and the repeatability with RSD≤0.59%(n=20)were obtained from this detection system.A total of 344 samples of wheat,corn,and flowers were selected,the correlation of chlorophyll a and b total correlations r between the diffuse reflectance and the conventional Arnon reached a very significant level.The diffuse reflectance could be used to detect glass emerald leaf with the thickness of about 3.0 mm.

关 键 词：3光源3光束 光学漫反射 叶绿素a和b 无损诊断技术 氮素 

分 类 号：S24[农业科学—农业电气化与自动化]