Molecular mechanism of Mimenghua Granules in treating dry eye  

作　　者：Dong-Hua Liu Peng-Fei Jiang Pei Liu Chen Ou Jun Peng Hou-Pan Song Qing-hua Peng 

机构地区：[1]Hunan University of Chinese Medicine,Changsha 410208,China [2]First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,China

出　　处：《Medical Data Mining》2021年第4期33-41,共9页TMR医学数据挖掘

基　　金：Projects supported by the National Natural Science Foundation of China(30772824,81574031);TCM Key Laboratory Construction Project of Hunan Province for Prevention and Treatment of ENT(2017TP1018);Central financial support for local university construction projects(2018-2019);State Administration of Traditional Chinese Medicine Ophthalmology Key Discipline Construction Project(ZK1801YK015);Key Discipline Construction Project of TCM Five Sense Organs in Hunan Province;Hunan Graduate Research Innovation Project(CX20200783).

摘　　要：Objective:To explore the molecular mechanism of Mimenghua Granules in treating dry eye based on network pharmacology and bioinformatics methods.Methods:Screening and prediction of possible blood-inducing active ingredients and action target of Mimenghua Granules through Traditional Chinese Medicine Systems Pharmacology database and analysis platform;mining dry eye-related diseases through disease gene database,gene target;use the functional protein combined network database STRING to draw the component-target and disease-target PPI networks,and extract the intersection of these two networks;use DAVID database analysis to screen key targets and analyze the mechanism of action.Results:A total of 593 active ingredients related to Mimenghua Granules were retrieved from the Traditional Chinese Medicine Systems Pharmacology database,and 59 blood active ingredients were obtained by screening based on pharmacokinetic parameters,and 680 targets related to these ingredients were retrieved;from disease genes,the database searches for 47 genes directly related to dry eye;3 key genes(ICAM1,IFNG,and IL-6)were obtained after the intersection of the component target and disease target PPI network;these genes are mainly involved in natural killer cell-mediated cytotoxicity,Jak-STAT signaling pathway,and Cytokine-cytokine receptor interaction.Conclusion:The mechanism of Mimenghua Granules in treating dry eye is related to the interaction of cytotoxic pathway,Jak-STAT signal pathway,and cytokines.The key gene targets are ICAM1,IFNG,and IL-6.

关 键 词：Mimenghua Granules Buddlejae Flos Lycii Fructus Chrysanthemi Flos Dry eye Network pharmacology BIOINFORMATICS 

分 类 号：TP3[自动化与计算机技术—计算机科学与技术]