分析和比较3种弧菌培养基的筛选性能  

The Screening Performance of Three Vibrio Culture Media was Analyzed and Compared

作　　者：梁苏育 袁峰 LIANG Suyu;YUAN Feng(Department of Microbiology,Jing'an District Center for Disease Control and Prevention,Shanghai 200072,China)

机构地区：[1]上海市静安区疾病预防控制中心微生物检验科,上海200072

出　　处：《中国卫生标准管理》2021年第23期120-123,共4页China Health Standard Management

摘　　要：目的分析和比较TCBS平板、mCPC平板和弧菌筛选显色平板等3种弧菌培养基的筛选性能,为快速准确确定致病性弧菌、提高检验质量提供可靠保证。方法通过将13株标准菌株,还有其中4株弧菌标准菌株的混合菌接种在TCBS平板、mCPC平板和弧菌筛选显色平板等3种弧菌培养基上培养,以及创伤弧菌和河流弧菌在弧菌筛选显色平板上再一次培养,观察它们的生长效果。结果 TCBS平板对致病性弧菌的选择范围很广,选择效率较高,是非特异性的,容易出现假阴阳性;mCPC平板对创伤弧菌的选择性最强,并且菌落典型单一,纯度高;弧菌筛选显色平板对副溶血性弧菌和溶藻弧菌的选择性强,活力强的创伤弧菌和河流弧菌才能在上面生长,它有较高的特异性,根据菌落颜色不同容易辨别。结论 TCBS平板适合样品中致病性弧菌的初步分离或者致病性弧菌含量较低的样品的分离;mCPC平板适合样品中创伤弧菌的分离,也适合样品中创伤弧菌菌株的纯化;弧菌筛选显色平板适合样品中副溶血性弧菌的分离,亦适合获取样品中活力强的创伤弧菌和河流弧菌。Objective Screenability of the TCBS plate, mCPC plate and Vibrio screening plate is analyzed and compared, which provides a reliable guarantee for the rapid and accurate determination of pathogenic Vibrio and the improvement of inspection quality. Methods By inoculating 13 standard strains, and a mixture of 4 standard vibrio strains among them on the TCBS plate, mCPC plate and Vibrio screening color plate, and inoculating again Vibrio vulnificus, and Vibrio fluvialis on the color screening plate of Vibrio, their growth effects was observed. Results TCBS plates had a wide selection range for pathogenic Vibrio, high selection efficiency, were non-specific and prone to false negative and positive;mCPC plate had the strongest selectivity to Vibrio vulnificus, and the bacterial colony was typically single with high purity;Vibrio screening color plate for Vibrio parahaemolyticus and Vibrio alginolyticus was highly selective, and only vibrio vulnificus and Vibrio fluvialis with strong vitality grew on it, it had high specificity and was easily identified according to the different color of the colony. Conclusion TCBS plate is suitable for the preliminary isolation of pathogenic Vibrio in samples or the isolation of samples with low content of pathogenic Vibrio;mCPC plate is suitable for the isolation of Vibrio vulnificus, and the purification of Vibrio vulnificus strains in samples;Vibrio screening color plate is suitable for the separation of Vibrio parahaemolyticus in the sample, and also suitable for the acquisition of vibrio vulnificus and Vibrio fluvialis with strong vitality in the sample.

关 键 词：副溶血性弧菌 创伤弧菌 溶藻弧菌 河流弧菌 TCBS平板 mCPC平板 弧菌筛选显色平板 

分 类 号：R446[医药卫生—诊断学]