In Vitro Propagation, Isolation and Expression Studies of Suaeda edulis Genes Involved in the Osmoprotectants Biosynthesis  

作　　者：Gloria Viviana Cerrillo-Rojas Mariana Tiscareno-Andrade Ana Erika Ochoa-Alfaro Eugenio Perez-Molphe Balch Ruth Elena Soria-Guerra Jose Francisco Morales-Dominguez 

机构地区：[1]Departamento de Quimica,Universidad Autonoma de Aguascalientes,Centro de Ciencias Basicas,Ciudad Universitaria,Aguascalientes,20131,Mexico [2]Facultad de Quimica,Universidad Autonoma de San Luis Potosi,Zona Universitaria,San Luis Potosi,78210,Mexico

出　　处：《Phyton-International Journal of Experimental Botany》2020年第3期715-726,共12页国际实验植物学杂志（英文）

基　　金：This research was financially supported by Universidad Autonoma de Aguascalientes(PIBT18-1);Consejo Nacional de Ciencia y Tecnologia(174795).

摘　　要：Halophytes are an excellent choice for the study of genes conferring salt tolerance to salt-sensitive plants and,they are suitable for reclamation and remediation of saline soil.We develop an in vitro plant propagation protocol and studies of genes involved with GB and Pro biosynthesis in Suaeda edulis.Axillary buds were used as explants and cultured in different treatments on Murashige and Skoog(MS)medium supplemented with different concentrations and combinations of plant growth regulators.The highest number of multiple shoots was on MS medium containing 1 mg/L Benzyladenine(BA)and/or 2 g/L activated carbon with 5.5±06 shoots per explant.The identification and expression analysis of genes involved in glycine betaine(GB)biosynthesis were S-adenosyl-methionine synthetase(SAMS),choline monooxygenase(CMO)and betaine alde-hyde dehydrogenase(BADH),and for proline(Pro)was pyrroline 5-carboxylate synthetase(P5CS).These sequences shared 90–95%of identity with others plant homologous in public databases.The amino acids sequence analysis showed that all these peptides contain some of the conserved motifs of those kinds of enzymes.The qRT-PCR analysis revealed a higher expression of SeBADH,SeCMO,and,SeP5CS genes in the roots and leaves from plants collected in the field in contrast with from in vitro plants.However,the expression level of SeSAMS was higher only in the leaves of plants collected in the field when com-pared to those cultivated in vitro.

关 键 词：Axillary buds HALOPHYTE PROLINE glycine betaine salt-resistant 

分 类 号：R73[医药卫生—肿瘤]