Ethanolic extract of Puhuang(Pollen Typhae) modulates lipopolysaccharide-induced inflammatory response through inducible nitric oxide synthase/cyclooxygenase-2 signaling in RAW 264.7 macrophages  

作　　者：CHEN Tzufan CHEN Chuntzu HUANG Yuanli BASKARAN Rathinasamy TSAI Jeffrey J.P. HU Rouhmei 

机构地区：[1]Department of Biotechnology,Asia University,Taichung 41354,China Taiwan [2]Department of Bioinformatics and Medical Engineering,Asia University,Taichung 41354,China Taiwan

出　　处：《Journal of Traditional Chinese Medicine》2021年第6期836-844,共9页中医杂志（英文版）

基　　金：Supported by Asia University grant number 104-ASIA-09 to R.M.H.;Ministry of Science and Technology of Taiwan(MOST)under contract numbers MOST 105-2632-E-468-002 and 109-2321-B-468-001 to J.P.T。

摘　　要：OBJECTIVE:To evaluate the immune modulatory response of Puhuang(Pollen Typhae),ethanolic extract of dried pollens(TP-E)and charcoal activated pollens(CTP-E)were used for their phytochemical evaluation and their modulatory response against lipopolysaccharide(LPS)induced inflammatory activity on RAW264.7 macrophage cells.METHODS:Biochemical assays were carried out to quantify the 1,1-Diphenyl-2-picrylhydrazyl Radical Scavenging Activity,Reducing Power,Ferrous ion chelating ability and total polyphenol content and flavonoids.Non-toxic dose of the extract(TP-E and CTP-E)was chosen based on 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.Effect of TP-E and CTP-E on lipopolysaccharides-induced inducible nitric oxide synthase(iNOS)and cyclooxygenase-2(COX-2)expression was measured by Western blot and quantitative PCR(q RT-PCR).Expression of inflammatory cytokines,such as interleukins(IL-1βand IL-6)and tumor necrosis factorα(TNF-α),was quantified using q RT-PCR.Mitogen-activated protein kinase pathway was analyzed using Western blot.RESULTS:Phytochemical analysis revealed that both TP-E and CTP-E have strong antioxidant activities and high flavonoid and phenolic contents.TP-E and CTP-E effectively inhibit the expression of i NOS and COX-2,thereby inhibiting its downstream proinflammatory regulators,the extracellular signal-related kinase-1/2,that decreases the expression of IL-1β,IL-6 and TNF-α.CONCLUSION:Phytochemical constituents present in Typha angustifolia Linn could be used for treating inflammation-related diseases.

关 键 词：Typha angustifolia LIPOPOLYSACCHARIDES inflammation NF-kappa B mitogen-activated protein kinases 

分 类 号：R285[医药卫生—中药学]