Effect of Roucongrong(Herba Cistanches Deserticolae) decoction on the substantia nigra through Wnt/β-catenin signaling pathway in rats with Parkinson's disease induced by 6-hydroxydopamine hydrochloride  

作　　者：XU Qian QIN Wei WU Fangzhen LIN Yao HONG Liting CHEN Dan HU Xuefeng CAI Jing 

机构地区：[1]Fujian Key Laboratory of Developmental and Neural Biology,College of Life Science,Fujian Normal University,Fuzhou 350117,China [2]College of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China [3]Department of Rheumatology and Endocrinology,The Second Affiliated Hospital of Fujian Traditional Chinese Medical University,Fuzhou 350003,China [4]Institute of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China

出　　处：《Journal of Traditional Chinese Medicine》2021年第5期762-770,共9页中医杂志（英文版）

基　　金：Supported by the Creation of Major New Drugs for Major National Science and Technology Projects(No.2019ZX09301154);National Natural Science Foundation of China(Nos.81904263,81804156);2021 Fujian Provincial Traditional Chinese Medicine Science and Technology Program;2018 Outstanding Young Scientific Research Project in Fujian province。

摘　　要：OBJECTIVE:To investigate the effect of Roucongrong(Herba Cistanches Deserticolae)decoction on the substantia nigra in rats with Parkinson’s disease(PD)induced by 6-hydroxydopamine hydrochloride(6-OHDA).To further determine whether the Wnt/β-catenin signaling pathway is involved in the action.METHODS:A rat model of PD was established by intracranial injection of 6-OHDA.Subsequently,three concentrations of Roucongrong(Herba Cistanches Deserticolae)decoction were prepared and administered to rats by gavage therapy for 14 d.Behavioral changes were measured in PD rats.In vivo tyrosine hydroxylase(TH)levels in the substantia nigra were examined by immunohistochemistry.Additionally,gene and protein expression levels of members of the Wnt/β-catenin signaling pathway were examined by Western blotting and polymerase chain reaction.Lastly,a Wnt/β-catenin inhibitor was used to investigate the mechanism of action in 1-methyl-4-phenylpyridinium(MPP+)-treated MES23.5 cells in vitro.RESULTS:Roucongrong(Herba Cistanches Deserticolae)decoction improved performance in the stride and gait adjustment tests in PD rats.It also increased TH in the substantia nigra and altered the expression of genes and proteins in the Wnt/β-catenin signaling pathway.Wnt/β-catenin inhibitor reduced the effect of Roucongrong(Herba Cistanches Deserticolae)decoction in MPP+-treated MES23.5 cells.CONCLUSION:Roucongrong(Herba Cistanches Deserticolae)decoction may promote neuronal survival in PD in vivo and in vitro by increasing TH content in the substantia nigra and by activating the Wnt/β-catenin signaling pathway.

关 键 词：CISTANCHE Parkinson disease SURVIVAL tyrosine 3-monooxygenase Wnt signaling pathway 

分 类 号：R285.5[医药卫生—中药学]