山奈酚的优化提取及生化性质  

Extraction Optimized of Kaempferol and Biochemical Properties

作　　者：张蕾[1] 王璐瑶 付学奇[2] 赵婉竹 郝婧玮[1] 王新颖 纪秋研 ZHANG Lei;WANG Luyao;FU Xueqi;ZHAO Wanzhu;HAO Jingwei;WANG Xinying;JI Qiuyan(College of Life Science and Technology,Mudanjiang Normal University,Mudanjiang 157011,Heilongjiang Province,China;School of Life Sciences,Jilin University,Changchun 130012,China;School of Public Health,Xinjiang Medical University,Urumqi 830017,China)

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157011 [2]吉林大学生命科学学院,长春130012 [3]新疆医科大学公共卫生学院,乌鲁木齐830017

出　　处：《吉林大学学报（理学版）》2022年第1期175-181,共7页Journal of Jilin University:Science Edition

基　　金：黑龙江省省属高校科研项目(批准号:1355ZD006);黑龙江省博士后科研启动项目(批准号:LBH-Q19190);黑龙江省大学生创新创业项目(批准号:202010233008).

摘　　要：通过双相酸水解法优化山奈酚提取条件,结合高效液相色谱(HPLC)法,通过单因素实验考察酸浓度、提取时间和固液比对山奈酚提取率的影响,并利用响应面Box-Behnken实验优化提取山奈酚的最佳工艺.实验结果表明:最佳提取条件的盐酸浓度为6.1×10^(-2) mmol/L,提取时间为12.87 min,固液比为1∶137.46,提取率达1.11%;山奈酚具有一定的抗氧化作用,对超氧自由基(·O^(2-))和1,1-二苯基-2-三硝基苯肼(DPPH·)均有一定的清除能力,尤其对DPPH·的清除能力更强,且清除能力随浓度的升高而提高.The extraction conditions of kaempferol were optimized by a two-phase acid hydrolysis method,and the effects of acid concentration,extraction time and solid-liquid ratio on the extraction yield of kaempferol were investigated by a single factor experiment combined with high performance liquid chromatography(HPLC).The response surface Box-Behnken experiment was used to optimize the extraction process of kaempferol.The experimental results show that the optimum extraction conditions are the concentration of hydrochloric acid of 6.1×10^(-2) mmol/L,the extraction time of 12.87 min,the solid-liquid ratio of 1∶137.46,and the extraction yield is 1.11%.Kaempferol has a certain antioxidant effect,and has certain scavenging ability on superoxide free radical(·O^(2-))and 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl(DPPH·),especially on DPPH·,and the scavenging ability increases with the increase of concentration.

关 键 词：山奈酚 酸水解 响应面法 生化性质 抗氧化 

分 类 号：Q50[生物学—生物化学] R93[医药卫生—生药学]