Met激酶抑制剂BMS-777607对舌鳞癌细胞CAL27增殖、凋亡的影响  

Effect of Met kinase inhibitor BMS-777607 on proliferation and apoptosis of tongue cell line CAL 27 squamous cell carcinoma

作　　者：马曌磊 刘佳楠 苏荣健 贺武斌 黄克强 MA Zhao-lei;LIU Jia-nan;SU Rong-jian;HE Wu-bin;HUANG Ke-qiang(The Second Affiliated Hospital and College of Stomatology of Jinzhou Medical University,Jinzhou 121004;Institute of Life Sciences,Jinzhou Medical University,Jinzhou 121001;The First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121001,Liaoning Province,China)

机构地区：[1]锦州医科大学附属第二医院·口腔医学院,辽宁锦州121004 [2]锦州医科大学生命科学研究院,辽宁锦州121001 [3]锦州医科大学附属第一医院,辽宁锦州121001

出　　处：《上海口腔医学》2021年第6期573-578,共6页Shanghai Journal of Stomatology

基　　金：辽宁省自然科学基金(2015020326)。

摘　　要：目的:探讨Met激酶抑制剂BMS-777607对人舌鳞状细胞癌(鳞癌)CAL27细胞增殖、凋亡的影响。方法:采用MTT、克隆形成实验及EdU细胞成像实验,检测BMS-777607对CAL27细胞增殖的影响。通过Heochst33342染色,观察BMS-777607对CAL27细胞的凋亡形态变化。采用JC-1染色,检测BMS-777607对CAL27细胞线粒体膜电位变化。采用Western免疫印迹检测BMS-777607对CAL27细胞中增殖蛋白Akt、p-Akt,凋亡相关蛋白Bcl-2、Cleaved caspase-3、Bax、Parp表达的影响。采用SPSS 22.0软件包对数据进行统计学分析。结果:BMS-777607抑制CAL27细胞增殖,促进其凋亡,呈浓度依赖性(P<0.05)。BMS-777607抑制Bcl-2、p-Akt表达,促进Bax、Cleaved caspase-3、Parp蛋白表达(P<0.05)。结论:BMS-777607能够抑制CAL27细胞增殖并促进其凋亡。PURPOSE:To investigate the effect of Met kinase inhibitor BMS-777607 on proliferation and apoptosis of tongue squamous cell carcinoma cell line CAL27.METHODS:The effect of BMS-777607 on proliferation of CAL27 was detected by MTT method,clone formation assay and EdU cell imaging.Morphological changes of apoptosis of CAL27 cells induced by BMS-777607 were observed by Heochst33342 staining.JC-1 staining was used to detect the changes of mitochondrial membrane potential of CAL27 cells treated with BMS-777607.Western blot was used to detect the effect of BMS-777607 on the expression of proliferation protein Akt,p-Akt and apoptosis-related proteins Bcl-2,Cleaved caspase-3,Bax and Parp in CAL27 cells.The data were analyzed using SPSS 22.0 software package.RESULTS:BMS-777607 inhibited proliferation and promoted apoptosis of CAL27 cells in a concentration-dependent manner(P<0.05).It also inhibited the expression of Bcl-2 and p-Akt and promoted the expression of Bax,Cleaved caspase-3 and Parp protein(P<0.05).CONCLUSIONS:BMS-777607 can inhibit proliferation and promote apoptosis of CAL27 cells.

关 键 词：Met激酶 BMS-777607 舌鳞癌 CAL27细胞 凋亡 

分 类 号：R739.8[医药卫生—肿瘤]