High quality genome sequences of thirteen Hypoxylaceae(Ascomycota)strengthen the phylogenetic family backbone and enable the discovery of new taxa  

作　　者：Daniel Wibberg Marc Stadler Christopher Lambert Boyke Bunk Cathrin Spröer Christian Rückert Jörn Kalinowski Russell JCox Eric Kuhnert 

机构地区：[1]Center for Biotechnology(CeBiTec),Bielefeld University,Universitätsstraße 27,33615 Bielefeld,Germany [2]Department Microbial Drugs,Helmholtz Centre for Infection Research(HZI),and German Centre for Infection Research(DZIF),Partner Site Hannover-Braunschweig,Inhoffenstrasse 7,38124 Braunschweig,Germany [3]Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures,Inhoffenstraße 7B,38124 Braunschweig,Germany [4]Centre of Biomolecular Drug Research(BMWZ),Institute for Organic Chemistry,Leibniz University Hannover,Schneiderberg 38,30167 Hannover,Germany

出　　处：《Fungal Diversity》2021年第1期7-28,共22页真菌多样性（英文）

基　　金：the DFG(Deutsche Forschungsgemeinschaft)priority program“Taxon-Omics:New Approaches for Discovering and Naming Biodiversity”(SPP 1991);The bioinformatics support of the BMBF-funded project‘Bielefeld-Gießen Center for Microbial Bioin-formatics;BiGi(Grant Number 031A533)’within the German Network for Bioinformatics Infrastructure(de.NBI)is gratefully acknowledged.

摘　　要：The Hypoxylaceae(Xylariales,Ascomycota)is a diverse family of mainly saprotrophic fungi,which commonly occur in angiosperm-dominated forests around the world.Despite their importance in forest and plant ecology as well as a prolific source of secondary metabolites and enzymes,genome sequences of related taxa are scarce and usually derived from envi-ronmental isolates.To address this lack of knowledge thirteen taxonomically well-defined representatives of the family and one member of the closely related Xylariaceae were genome sequenced using combinations of Illumina and Oxford nanopore technologies or PacBio sequencing.The workflow leads to high quality draft genome sequences with an average N50 of 3.0 Mbp.A backbone phylogenomic tree was calculated based on the amino acid sequences of 4912 core genes reflecting the current accepted taxonomic concept of the Hypoxylaceae.A Percentage of Conserved Proteins(POCP)analysis revealed that 70%of the proteins are conserved within the family,a value with potential application for the definition of family boundaries within the order Xylariales.Also,Hypomontagnella spongiphila is proposed as a new marine derived lineage of Hypom.monticulosa based on in-depth genomic comparison and morphological differences of the cultures.The results showed that both species share 95%of their genes corresponding to more than 700 strain-specific proteins.This difference is not reflected by standard taxonomic assessments(morphology of sexual and asexual morph,chemotaxonomy,phylogeny),preventing species delimitation based on traditional concepts.Genetic changes are likely to be the result of environmental adaptations and selective pressure,the driving force of speciation.These data provide an important starting point for the establishment of a stable phylogeny of the Xylariales;they enable studies on evolution,ecological behavior and biosynthesis of natural products;and they significantly advance the taxonomy of fungi.

关 键 词：AAI ANI Cazyme Oxford nanopore PHYLOGENOMICS POCP XYLARIALES 

分 类 号：TP3[自动化与计算机技术—计算机科学与技术]