铜(Ⅱ)配合物{[Cu(OMBA)_(2)]_(2)·(DMF)_(2)}的合成、CT-DNA结合及细胞毒性  

Synthesis,CT-DNA Binding,and Cytotoxic Activity of Copper(Ⅱ)Complex{[Cu(OMBA)_(2)]_(2)·(DMF)_(2)}

作　　者：曾振芳[1] 蔡杰慧 黄秋萍[1] 韦友欢[1] 陈丽苗 黄秋婵[1] ZENG Zhenfang;CAI Jiehui;HUANG Qiuping;WEI Youhuan;CHEN Limiao;HUANG Qiuchan(School of Chemical and Biological Engineering, Guangxi Normal University for Nationalities, Chongzuo 532200, China)

机构地区：[1]广西民族师范学院化学与生物工程学院,崇左532200

出　　处：《人工晶体学报》2021年第12期2300-2306,2331,共8页Journal of Synthetic Crystals

基　　金：广西教育厅高校科研项目(2017KY0846,2019KY0769);广西民族师范学院科研项目(327003070414,327002060107,31700702,2019YB018,2020GP008)。

摘　　要：合成了配合物{[Cu(OMBA)_(2)]_(2)·(DMF)_(2)}(OMBA=邻甲基苯甲酸,DMF=N,N-二甲基甲酰胺),配合物结构经元素分析、IR和X射线单晶衍射表征,该配合物属于三斜晶系,P1空间群,晶胞参数为a=1.0370(5)nm,b=1.0536(4)nm,c=1.1059(5)nm,α=62.737(6)°,β=73.355(7)°,γ=63.231(7)°,Z=1,D_(c)=1.416 g·cm^(-3),F(000)=422,最终结构残差因子R1=0.0515,wR_(2)=0.1404。采用紫外、荧光和黏度法研究了配合物和小牛胸腺DNA(CT-DNA)的相互作用方式。结果表明,配合物与DNA的结合常数K_(b)=939.61 L·mol^(-1),K_(sv)=3.00×10^(3) L·mol^(-1),猝灭速率常数K_(q)=3.00×10^(11) L·mol^(-1)·s^(-1)。配合物静态猝灭CT-DNA的荧光,结合常数K_(a)=5.38×10^(3) L·mol^(-1),结合位点n=1,配合物对胃癌细胞A549、宫颈癌细胞Hela和肝癌细胞HepG2有抗增殖作用。In this paper,a copper(Ⅱ)complex{[Cu(OMBA)_(2)]_(2)·(DMF)_(2)}(OMBA=o-methylbenzoic acid,DMF=N,N-dimethylformamide)was synthesized.The structure of the complex was characterized using elemental analysis,infrared(IR)and X-ray single crystal diffraction.The complex belongs to triclinic,P1 space group,with a=1.0370(5)nm,b=1.0536(4)nm,c=1.1059(5)nm,α=62.737(6)°,β=73.355(7)°,γ=63.231(7)°,Z=1,D_(c)=1.416 g·cm^(-3),F(000)=422 unit cell parameters.The final R1=0.0515,wR2=0.1404.The interaction between CT-DNA(calf thymus DNA)and the complex was evaluated by UV-Vis,fluorescence spectroscopy,and viscometry.When the complex bound to CT-DNA,the binding constant K_(b)=939.61 L·mol^(-1),K_(sv)=3.00×10^(3) L·mol^(-1),and K_(q)=3.00×10^(11) L·mol^(-1)·s^(-1).The complex quenched the intrinsic fluorescence of CT-DNA via static quenching,K_(a)=5.38×10^(3) L·mol^(-1),n=1.The complex has anti-proliferation effects on A549,Hela and HepG2.

关 键 词：铜(Ⅱ) 配合物 合成 DNA结合 细胞毒性 抗肿瘤活性 DNA复制 

分 类 号：O614.1[理学—无机化学]