肠型脂肪酸结合蛋白、二胺氧化酶在绞窄性肠梗阻大鼠体内与肠管损伤的相关性  

Correlation of intestinal fatty acid binding protein and diamine oxidase with intestinal injury in strangulated bowel obstruction mice

作　　者：张剑 徐俊 洪丽华 张秋玲[2] Zhang Jian;Xu Jun;Hong Lihua;Zhang Qiuling(Department of General Surgery,the First People’s Hospital of Hangzhou Lin An District,Hangzhou 311300,China)

机构地区：[1]浙江省杭州市临安区第一人民医院普外1科,311300 [2]杭州师范大学附属医院内分泌科,310015

出　　处：《中华内分泌外科杂志》2021年第6期598-602,共5页Chinese Journal of Endocrine Surgery

基　　金：浙江省医药卫生科技计划项目(2020KY713);杭州市科学技术委员会项目(20171226Y250)。

摘　　要：目的探究肠型脂肪酸结合蛋白、二胺氧化酶在绞窄性肠梗阻大鼠体内与肠管损伤的相关性。方法选取160只SD大鼠,采用随机数表法分为5组,A组(正常对照组)、B组(假手术组)、C组(单纯性肠梗阻组)、绞窄性肠梗阻组分为D组(急性肠系膜上动脉缺血组)和E组(急性肠系膜动静脉血运障碍组),每组32只。除A组外,其余各组均手术造模。A组麻醉后取静脉血和小肠肠段,B~E组于造模术后4 h取静脉血和小肠肠段。从大鼠静脉血中提取得到血清样本,肠段浸泡得到肠液样本。观察肠段并评估肠管损伤,检测血清和肠液肠型脂肪酸结合蛋白(I-FABP)水平、二胺氧化酶(DAO)活性。Pearson相关性分析用于分析肠管损伤与血清和肠液I-FABP水平、DAO活性的相关性。结果B~E组肠管损伤评分均高于A组,C~E组肠管损伤评分均高于B组,D、E组肠管损伤评分均高于C组,E组肠管损伤评分均高于D组(P<0.05)。C~E组血清I-FABP水平和DAO活性均高于A组和B组,D、E组血清I-FABP水平和DAO活性均高于C组(P<0.05)。C~E组肠液I-FABP水平和DAO活性均高于A组和B组,D、E组肠液I-FABP水平和DAO活性均高于C组(P<0.05)。肠管损伤评分与血清和肠液I-FABP水平、DAO活性均呈正相关(r=0.972,P<0.001;r=0.899,P<0.001;r=0.961,P<0.001;r=0.828,P<0.001)。结论绞窄性肠梗阻大鼠的肠管损伤与肠道缺血有关。绞窄性肠梗阻大鼠体内存在血清和肠液I-FABP水平、DAO活性升高,且血清和肠液I-FABP水平、DAO活性与肠管损伤程度密切相关。Objective To investigate the correlation of intestinal fatty acid binding protein and diamine oxidase with intestinal injury in strangulated bowel obstruction mice.Methods 160 SD rats were divided into 5 groups by random number table:group A(n=32):normal control group;group B(n=32):sham operation group;group C(n=32):simple intestinal obstruction group;The strangulated intestinal obstruction group was divided into group D(n=32):acute superior mesenteric artery ischemia group and group E(n=32):acute mesenteric arterial and venous ischemia.Except group A,other groups were given operation for modeling.Venous blood and small intestinal segment of group A was collected after anaesthesia,and venous blood and small intestinal segment of other groups were collected after modeling for 4 h.Serum samples were collected from venous blood,and intestinal fluid samples were collected by soaking the small intestinal segments.The intestinal segments were observed and the intestinal injury was evaluated.The levels of intestinal fatty acid binding protein(I-FABP)and activity of diamine oxidase(DAO)in serum and intestinal fluid were detected.Pearson correlation analysis was used to analyze the correlation between intestinal injury and the serum and intestinal fluid levels of I-FABP and activity of DAO,respectively.Results The intestinal damage scores in group B,C,D and E were higher than that in group A,the intestinal damage scores in groups C,D and E were higher than that in group B,the intestinal damage scores in groups D and E were higher than that in group C,and the intestinal damage score in group E was higher than that in group D(P<0.05).The serum I-FABP level and DAO activity in group C,D and E were higher than those in group A and B,and the serum I-FABP level and DAO activity in group D and E were higher than those in group C(P<0.05).The level of I-FABP and DAO activity in intestinal fluid in group C,D and E were higher than those in groups A and B,and the level of I-FABP and DAO activity in intestinal fluid in group D and E

关 键 词：绞窄性肠梗阻 大鼠 肠型脂肪酸结合蛋白 二胺氧化酶 肠管损伤 

分 类 号：R574.2[医药卫生—消化系统]