长双歧杆菌F16的鉴定及益生性研究  

Identification and probiotic study of Bifidobacterium longum F16

作　　者：冯诗诗 惠明[1] 杨林英 田青[1] 胡军[2] FENG Shishi;HUI Ming;YANG Linying;TIAN Qing;HU Jun(College of Biological Engineering,Henan University of Technology,Zhengzhou 450001,China;Basic Medical College,Zhengzhou University,Zhengzhou 450001,China)

机构地区：[1]河南工业大学生物工程学院,河南郑州450001 [2]郑州大学基础医学院,河南郑州450001

出　　处：《河南工业大学学报（自然科学版）》2021年第6期65-70,120,共7页Journal of Henan University of Technology：Natural Science Edition

基　　金：中国工程科技发展战略河南研究院战略咨询研究项目(2020HENZT13)。

摘　　要：双歧杆菌是一种严格厌氧的典型肠道益生菌,研究双歧杆菌益生性能对人体健康具有十分重要的意义。从菌样中分离出1株具有一定耐氧性的双歧杆菌F16,通过16S rRNA测序和构建系统发育树,鉴定菌株F16为长双歧杆菌菌属(Bifidobacterium longus)。以F16为出发菌株,采用HPLC法、比色法、平板计数法及模拟人工胃肠道环境等对其乳酸产量、降胆固醇能力、耐酸性、耐胆盐性及对人工胃肠液的耐受性等进行了研究。结果表明:F16菌株代谢产乳酸量为10.63 mg/mL;胆固醇降解率在46%-75%之间,降解胆固醇的效果显著,72 h的降解率明显高于48 h的;对酸度的耐受性较好,在人工胃肠道模拟环境中存活率平均达到85.20%;在0-3%胆盐条件下,比严格厌氧的双歧杆菌耐受性更强。F16菌株具有良好的益生特性,对开发新一代双歧杆菌制品具有潜在的应用价值。Bifidobacterium is a typical intestinal probiotics of strictly anaerobic, and studying its probiotic properties is very important for human health. Through 16 S rRNA sequencing and phylogenetic tree construction, a strain of Bifidobacterium longum with certain oxygen tolerance was isolated and identified(named Bifidobacterium longus F16). In addition, through morphological identification and physiological biochemical identification, this strain has the following characteristics: neat colony edge, convex, smooth and opaque, milky or gray surface, catalase is negative, fructose-6-phosphate phosphatase is positive, the strain can decompose lactose, fructose, maltose, and produce acid. By means of HPLC, colorimetric method, plate counting method, and simulated gastrointestinal environment, the content of lactic acid, the ability to degrade cholesterol(1% and 5% inoculum), the acid resistance, bile salt resistance and the tolerance to artificial gastrointestinal fluid of this strain were studied. The concentration of lactic acid produced by the strain was 10.63 mg/mL. The degradation rate of cholesterol was between 46-75%, suggesting that a significant cholesterol degradation effect, and this degradation was in a time-dependent manner. The tolerance curve to acidity was more gentle, the acid resistance was better, and the survival rate was also high(85.20%) in simulated gastrointestinal simulation environment. At 0-3% bile salt concentration, the tolerance was stronger than that of strictly anaerobic Bifidobacterium. As a result, F16 has excellent probiotic properties and has important application value for the development of a new generation of Bifidobacterium products.

关 键 词：双歧杆菌 益生菌 耐氧性 胆固醇降解 

分 类 号：TS201.3[轻工技术与工程—食品科学]