不同水平自噬对替莫唑胺抑制神经胶质瘤细胞增殖的影响  

Effects of different autophagy level on temozolomide-induced proliferation inhibition in glioma cells

作　　者：汪滨[1] 王青霞[2] 虞欢东 于海涛[1] WANG Bin;WANG Qingxia;YU Huandong;YU Haitao(Department of Cerebral Surgery,Hangzhou Xiaoshan TCM Hospital(Jiangnan Hospital Affiliated to Zhejiang Chinese Medical University),Hangzhou 311201,China)

机构地区：[1]杭州市萧山区中医院(浙江中医药大学附属江南医院)脑外科,311201 [2]杭州市萧山区中医院(浙江中医药大学附属江南医院)药剂科,311201

出　　处：《浙江医学》2021年第24期2623-2626,共4页Zhejiang Medical Journal

摘　　要：目的探讨不同水平自噬对替莫唑胺抑制神经胶质瘤细胞增殖的影响。方法使用0(对照)、10、25、50μg/L的雷帕霉素(RAPA)预处理人胶质瘤细胞(U87细胞)0.5 h;再加入200μmol/L替莫唑胺作用24 h,收集U87细胞。采用流式细胞术检测自噬标志物微管相关轻链蛋白3(LC3)-Ⅱ表达量,RT-PCR法检测自噬相关基因Beclin-1 mRNA相对表达量,MTT法检测细胞增殖抑制率。结果对照组、10μg/L组、25μg/L组和50μg/L组U87细胞LC3-Ⅱ表达量、Beclin-1 mRNA相对表达量及细胞增殖抑制率比较,差异均有统计学意义(均P<0.05);经两两比较发现,随着RAPA浓度的增加,LC3-Ⅱ表达量及Beclin-1 mRNA相对表达量均逐渐升高(均P<0.05),而细胞增殖抑制率先下降后逐步升高(均P<0.05)。结论不同水平自噬对替莫唑胺抑制神经胶质瘤细胞增殖产生不同的影响,随着自噬水平的提高,替莫唑胺对神经胶质瘤细胞的增殖抑制率先下降后逐渐升高。Objective To investigate the effects of different autophagy level on the temozolomide-induced proliferation inhibition in glioma cells.Methods Human glioma U87 cells were pretreated with rapamycin at concentration of 0(control group),10,25,50μg/L for 0.5 h,then treated with 200μmol/L temozolomide for 24 h.The expression level of autophagy marker microtubule-associated light chain protein 3(LC3)-Ⅱwas detected by flow cytometry,the relative expression level of autophagy related gene Beclin-1 mRNA was detected by RT-PCR,and the inhibition rate of cell proliferation was detected by MTT assay.Results There were significant differences in expression levels of LC3-Ⅱand beclin-1 mRNA and inhibition rate of U87 cell proliferation among 4 groups(all P<0.05).Compared in pairs,the expression levels of LC3-Ⅱand beclin-1 mRNA were elevated with the increase of RAPA concentration(all P<0.05),while the inhibition of cell proliferation decreased firstly and then increased gradually(all P<0.05).Conclusion The inhibitory effects of temozolomide on proliferation of glioma cells are associated with the levels of rapamycine-induced cell autophagy.

关 键 词：自噬 替莫唑胺 神经胶质瘤细胞 雷帕霉素 细胞增殖 

分 类 号：R739.41[医药卫生—肿瘤]