TKTL1和WISP-1在喉癌患者病灶组织中的表达及对预后的影响  

Expression of TKTL1 and WISP-1 in laryngeal carcinoma tissues and the influence on prognosis

作　　者：魏鑫鑫[1] 李晓玲 黄维平[1] 尹中普[1] WEI Xinxin;LI Xiaoling;HUANFG Weiping;YIN Zhongpu(Department of Otolaryngology Head and Neck Surgery,Nanyang Central Hospital,Nanyang,Henan,China,473000)

机构地区：[1]南阳市中心医院耳鼻咽喉头颈外科,河南南阳473000

出　　处：《分子诊断与治疗杂志》2021年第12期1977-1980,1985,共5页Journal of Molecular Diagnostics and Therapy

基　　金：河南省科技攻关项目(16210213135)。

摘　　要：目的探究喉癌患者病灶组织中转酮醇酶样基因1(TKTL1)和Wnt1诱导分泌蛋白1(WISP-1)的表达水平,分析其与患者预后的关系。方法选取2016年3月至2018年3月于本院接受治疗的喉鳞状细胞癌患者75例为喉癌组、声带息肉患者68例为对照组,比较两组患者TKTL1和WISP-1的阳性表达率、TKTL1 mRNA表达量、WISP-1蛋白表达水平、血清肿瘤标志物和癌细胞增殖活力,Pearson法分析TKTL1、WISP-1表达与血清肿瘤标志物、癌细胞增殖活力指标的相关性,Kaplan-Meier法分析TKTL1、WISP-1表达与患者预后的关系。结果喉癌组的TKTL1、WISP-1阳性表达率,TKTL1 mRNA、WISP-1蛋白、血清癌胚抗原(CEA)、糖类抗原199(CA199)表达水平,组织中增殖基因信号传导及转录激活因子3(STAT3)、原癌基因c-myc的mRNA表达量均明显高于对照组,差异均有统计学意义(P<0.05)。TKTL1、WISP-1的表达量与CEA、CA199、STAT3 mRNA和c-myc mRNA表达均呈明显正相关(P<0.05)。TKTL1、WISP-1高表达患者的3年累计生存率明显高于TKTL1、WISP-1低表达患者,差异均有统计学意义(P<0.05)。结论 TKTL1、WISP-1在喉癌患者病灶组织中呈高表达状态,可促进癌细胞增殖,影响患者的术后生存时间。Objective To explore the expression levels of transketolase like 1(TKTL1)and Wntinduced secreted protein-1(WISP-1)in laryngeal carcinoma tissues and analyze their relationship with the prognosis. Methods Totally 75 patients with laryngeal squamous cell carcinoma who were treated with operation in the hospital were selected as the laryngeal carcinoma group from March 2016 to March 2018,68 patients with vocal cord polyps were selected as the control group. The positive expression rates of TKTL1 and WISP-1,expression levels of TKTL1 m RNA and WISP-1,Serum tumor markers and proliferating activity of cancer cells in the two groups were compared. Pearson method was used to analyze the correlation of the expression of TKTL1 and WISP-1 with serum tumor markers and proliferating activity of cancer cells. KaplanMeier method was used to analyze the relationship between the expression of TKTL1 and WISP-1 and patient prognosis. Results The positive expression rates of TKTL1 and WISP-1,expression levels of TKTL1 m RNA and WISP-1,levels of serum carcinoembryonic antigen(CEA)and carbohydrate antigen 199(CA199),expression levels of signal transducer and activator of transcription 3(STAT3)m RNA and proto-oncogene(c-myc)in the laryngeal carcinoma group were significantly higher than those in the control group(P<0.05).The expression levels of TKTL1 and WISP-1 were positively correlated with the expression of CEA,CA199,STAT3 m RNA and c-myc m RNA(P<0.05). The 3-year cumulative survival rates of patients with high expression of TKTL1 and WISP-1 were significantly higher than those of patients with low expression of TKTL1 and WISP-1(P<0.05). Conclusion TKTL1 and WISP-1 are highly expressed in laryngeal carcinoma tissues,which promotes the proliferation of cancer cells and affects the patients. survival time after surgery.

关 键 词：喉鳞状细胞癌 转酮醇酶样基因1 Wnt1诱导分泌蛋白1 肿瘤标志物 

分 类 号：R739.65[医药卫生—肿瘤]