芹菜素对帕金森模型细胞氧化应激水平的影响及机制的研究  

Effect of apigenin on oxidative stress in Parkinson’s disease model cells and its mechanism

作　　者：张跃其[1] 胡炳蕾 王默然[3] 马海强[4] 张君 陈学丛 ZHANG Yueqi;HU Binglei;WANG Moran;MA Haiqiang;ZHANG Jun;CHEN Xuecong(Department of Neurology,Weifang People’s Hospital,Weifang 261000,China;Department of Gynecology,Weifang Hospital of Traditional Chinese Medicine,Weifang 261000;Department of Geriatrics,Weifang Hospital of Traditional Chinese Medicine,Weifang 261000;Department of Orthopedics,Weifang Hospital of Traditional Chinese Medicine,Weifang 261000;Shandong Department of Neurology,First Affiliated Hospital of First Medical University,Jinan 250013)

机构地区：[1]潍坊市人民医院神经内科,山东潍坊261000 [2]潍坊市中医院妇科,山东潍坊261000 [3]潍坊市中医院老年科,山东潍坊261000 [4]潍坊市中医院骨科,山东潍坊261000 [5]山东第一医科大学第一附属医院神经内科,济南250013

出　　处：《中国比较医学杂志》2021年第12期77-83,共7页Chinese Journal of Comparative Medicine

基　　金：潍坊市卫生和计划生育委员会中医药科研项目(2018-1-006)。

摘　　要：目的研究芹菜素(AGN)对MPP;诱发的帕金森细胞模型中自噬、氧化应激的影响及神经保护作用。方法CCK-8筛选AGN有效浓度,SH-SY5Y细胞分6组:(1)对照组;(2)MPP;组;(3)A组:AGN浓度10μmol/L;(4)B组:AGN浓度20μmol/L;(5)C组:AGN浓度40μmol/L;(6)D组:AGN浓度100μmol/L。确定AGN有效浓度后将细胞分为3组:对照组、MPP;组及AGN^(+)MPP;组。检测细胞凋亡及Caspase3、Bcl-2、Bax、LC3-II、LC3-I、Beclin-1、ULK1蛋白表达,检测丙二醇、超氧化物歧化酶和谷胱甘肽过氧化物酶水平。分析每组GFP-LC3斑点。结果AGN在40μmol/L时能显著降低MPP;诱导的细胞毒性损伤,选择40μmol/L作为最佳药物保护浓度进行后续研究。AGN^(+)MPP;组细胞凋亡率较MPP;组下降(P<0.05)。相较于MPP;组,AGN^(+)MPP;组Caspase3表达降低,Bcl-2/Bax值升高(P值均<0.01)。与MPP;组比较,AGN^(+)MPP;组抑制MDA的表达(P<0.05),恢复GPX和SOD的活性(P<0.05)。AGN^(+)MPP;组细胞内GFP-LC3斑点较MPP;组显著减少(P<0.05)。对比MPP;组,AGN^(+)MPP;组LC3-II/LC3-I比值及Beclin-1、ULK1表达显著降低(P值均<0.05)。结论AGN能减轻MPP;诱导的SH-SY5Y细胞凋亡,增强抗氧化能力,其细胞保护作用可能与降低细胞自噬水平相关。Objective To study the effect of apigenin(AGN)on autophagy and oxidative stress,and its neuroprotection in MPP;-induced Parkinson’s model cells.Methods CCK-8 assays were used to screen the effective concentration of AGN.SH-SY5 Y cells were divided into six groups:(1)Control group;(2)MPP;group;(3)group A:10μmol/L AGN;(4)group B:20μmol/L AGN;(5)group C:40μmol/L AGN;(6)group D:100μmol/L AGN.After determining the effective concentration of AGN,the cells were divided into three groups:Control,MPP;,and AGN^(+)MPP;groups.Apoptosis,protein expression of Caspase 3,Bcl-2,Bax,LC3-II,LC3-I,Beclin-1,and ULK-1,and propylene glycol,superoxide dismutase,and glutathione peroxidase levels were examined.Analyze each group of GFP-LC3 spots.Results AGN at 40μmol/L significantly reduced the cytotoxic damage induced by MPP;.Therefore,40μmol/L AGN was chosen as the optimal protective concentration for subsequent experiments.The apoptosis rate of the AGN^(+)MPP;group was lower than that of the MPP;group(P<0.05).Compared with the MPP;group,expression of Caspase 3 was decreased and the the Bcl-2/Bax ratio was increased in the AGN^(+)MPP;group(all P<0.01).Compared with the MPP;group,expression of MDA was inhibited(P<0.05)and the activities of GPX and SOD were restored in the AGN^(+)MPP;group(P<0.05).Intracellular GFP-LC3 spots in the AGN^(+)MPP;group were significantly reduced compared with those in the MPP;group(P<0.05).Compared with the MPP;group,the LC3-II/LC3-I ratio and expression of Beclin-1 and ULK1 were significantly reduced in the AGN^(+)MPP;group(all P<0.05).Conclusions AGN alleviates apoptosis of SH-SY5 Y cells induced by MPP;and enhances their antioxidant capacity.Its cytoprotective effect may be related to reduction of autophagy.

关 键 词：芹菜素 自噬 氧化应激 凋亡 

分 类 号：R-33[医药卫生]