Epicatechin gallate prevents the de novo synthesis of fatty acid and the migration of prostate cancer cells  

作　　者：Luyao Chen Yaping Guo Zixuan Wu Shuwu Zhao Zhaiyi Zhang Fang Zheng Likang Sun Zheng Hao Chen Xu Tao Wang Yanfei Peng 

机构地区：[1]School of Integrative Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China [2]Institute of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China [3]Department of Colorectal Surgery,Tianjin Union Medical Center,Tianjin 300121,China

出　　处：《Acta Biochimica et Biophysica Sinica》2021年第12期1662-1669,共8页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China(Nos.81703828,81873100 and 81673732);the research and innovation project of graduate students in the Tianjin University of Traditional Chinese Medicine(No.YJSKC-20191023).

摘　　要：Lipid metabolism disorder caused by the upregulation of lipogenic genes is a typical feature of prostate cancer.The synthesis of fatty acids is enhanced to accelerate the development of prostate cancer and is considered as a potential therapeutic target.Epicatechin gallate,an active compound of green tea,has been reported to modulate lipid metabolism.In this research,the potential role of epicatechin gallate in prostate cancer cells was evaluated.The results indicated that epicatechin gallate downregulates the expression of acetyl-CoA carboxylase,ATP citrate lyase,and fatty acid synthase in prostate cancer cells and prostate xenograft tissues,suggesting that epicatechin gallate can inhibit de novo fatty acid synthesis.Moreover,epicatechin gallate significantly restrains the migration rather than the viability of prostate cancer cells.PI3K/AKT/mTOR signaling pathway,which exhibits regulatory effect on lipogenesis,is also inhibited under epicatechin gallate treatment,while pretreatment with AKT activator SC79 or mTOR activator MHY1485 blocks the inhibitory effect of epicatechin gallate on the expression of lipogenic genes and the migration of prostate cancer cells.In conclusion,this study revealed that epicatechin gallate impairs the synthesis of fatty acids via inhibition PI3K/AKT/mTOR signaling pathway and then attenuates the migration of prostate cancer cells.

关 键 词：epicatechin gallate fatty acid synthesis MIGRATION prostate cancer 

分 类 号：R737.25[医药卫生—肿瘤]