蜂王浆对免疫功能低下小鼠免疫功能的影响  

Effect of royal jelly on mice with immunodeficiency

作　　者：李丹 王文千 李明亮 方小明[1] 彭文君[1] 田文礼[1] 魏颖[2] LI Dan;WANG Wenqian;LI Mingliang;FANG Xiaoming;PENG Wenjun;TIAN Wenli;WEI Ying(Institute of Apicultural Research,CAAS,Beijing 100093,China;China National Research Institute of Food&Fermentation Industries,Beijing Engineering Research Center of Protein and Functional Peptides,Beijing 100015,China)

机构地区：[1]中国农业科学院蜜蜂研究所,北京100093 [2]中国食品发酵工业研究院,北京市蛋白功能肽工程技术研究中心,北京100015

出　　处：《食品与发酵工业》2022年第1期139-145,共7页Food and Fermentation Industries

基　　金：国家自然科学基金项目(31972087);国家现代农业(蜜蜂)产业技术体系建设专项(NCYTI-43-KXJ17);中国农业科学院科技创新工程项目(CAAS-ASTIP-2015-IAR)。

摘　　要：研究蜂王浆对免疫低下小鼠免疫系统的调节作用。48只雄性昆明小鼠随机分为空白组、环磷酰胺模型组、酪蛋白组以及蜂王浆低、中、高剂量组,每组8只。除空白组外,其余5组每天腹腔注射80 mg/(kg bw)环磷酰胺,建立免疫低下小鼠模型。各组小鼠经不同样品连续灌胃30 d后测量小鼠体重、脾脏和胸腺质量及HE染色观察脾脏与肝脏的形态、白细胞计数、免疫球蛋白含量、细胞因子水平、脾淋巴细胞增殖能力、睾酮含量。造模后,脾脏和胸腺指数降低,小鼠脾脏和肝脏镜下形态学结构紊乱,白细胞计数、免疫球蛋白、IL-6水平显著降低,T/B淋巴细胞增殖能力显著下降;经蜂王浆干预后,相比于模型组,蜂王浆干预组小鼠上述指标有不同程度的改善,其中以高剂量组效果最为显著。蜂王浆可以增强环磷酰胺造成的免疫功能低下小鼠的免疫功能。The aim of this study was to explore the effect of royal jelly on immunocompromised mice.Forty-eight male Kunming mice were randomly divided into blank group,cyclophosphamide model group,casein group as well as royal jelly low-dose,medium-dose and high-dose groups,with eight mice in each group.Except for the blank group,the other five groups were intraperitoneally injected with 80 mg/(kg bw)cyclophosphamide every day to establish immunocompromised mouse models.After 30 d of continuous gavage,the body weight,spleen and thymus weight,morphology of spleen were measured.Meanwhile the morphology of spleen and liver,white blood cell count,immunoglobulin content,cytokine level,splenic lymphocyte proliferation ability and testosterone content were observed and by Hematoxylin-Eosin staining.After modeling,the spleen and thymus index were decreased,the morphological structure under the microscope of spleen and liver of mice was disordered.The WBC count,immunoglobulin,IL-6 levels and the proliferation ability of T/B lymphocyte were significantly decreased.However,after the royal jelly intervention,above-mentioned indexes were improved to different extent,and the effect was most significant in the high-dose group.Royal jelly can enhance the immune function of mice with immunodeficiency caused by cyclophosphamide.

关 键 词：蜂王浆 免疫低下 免疫调节 环磷酰胺 

分 类 号：S896.3[农业科学—特种经济动物饲养]