口腔鳞癌相关巨噬细胞对淋巴管生成的影响  

Effect of OSCC-associated macrophages on the lymphangiogenesis

作　　者：孙海滨 刘颜彬[1] 王博源 秦力铮[1] 邢汝东[1] 韩正学[1] SUN Hai-bin;LIU Yan-bin;WANG Bo-yuan;QIN Li-Zheng;XING Ru-dong;HAN Zheng-xue(Department of Oral and Maxillofacial-Head and Neck Oncology,Capital Medical University School of Stomatology,Beijing 100050,China)

机构地区：[1]首都医科大学口腔医学院颌面头颈肿瘤科,北京100050

出　　处：《北京口腔医学》2021年第6期333-337,共5页Beijing Journal of Stomatology

基　　金：北京口腔医院学科建设基金基础专项(19-09-07);首都医科大学科研培育基金(PYZ19077)。

摘　　要：目的探讨口腔鳞癌相关巨噬细胞对淋巴管生成的影响。方法体外诱导人单核细胞(THP-1形成M0巨噬细胞,后与CAL27细胞共培养后分化生成OSCC相关巨噬细胞,实时荧光定量PCR法(real-time PCR)检测诱导前后THP-1细胞中M2型巨噬细胞相关因子CD68和CD206的表达差异。淋巴管成管实验检测OSCC相关巨噬细胞上清液对人淋巴管内皮细胞(human lymphatic endothelial cells,HLEC)成管数量的影响,进一步通过小干扰RNA(small interfering RNA,siRNA)沉默血管内皮生长因-C(vascular endothelial growth factor-C,VEGF-C)基因表达的方法检测其在淋巴管生成中的重要作用。结果经佛波脂诱导后的THP-1能够分化形成M0巨噬细胞,且与CAL27细胞共培养后,CD68和CD206的表达水平明显增高(P<0.01)。与对照组相比,OSCC相关巨噬细胞上清能明显刺激淋巴管生成(P<0.001),而转染VEGF-C-siRNA后,其成管数量明显减少(P<0.001)。结论 OSCC相关巨噬细胞能够促进淋巴管的生成,其可能是通过分泌VEGF-C发挥相关作用。Objective To investigate the effect of OSCC-associated macrophages on the lymph angiogenesis.Methods Human monocytes(THP-1)were induced to form M0 macrophages in vitro,and then co-cultured with the CAL27 cells to differentiate into OSCC-associated macrophages,Real-time PCR was performed to evaluate the expression of M2 macrophage related factors CD68 and CD206.The effect of OSCC-associated macrophages on lymphangiogenesis was detected by the tube formation assays of HLEC in different culture supernatants,and the role of VEGF-C in lymphangiogenesis was detected by siRNA silencing.Results THP-1 cells induced by phorbol myristate acetate could differentiate into M0 macrophages,and the expression levels of CD68 and CD206 were significantly up-regulated after cocultured with CAL27 cells(P<0.01).Conditioned medium from OSCC-associated macrophages could significantly activate lymphangiogenesis compared to the control group(P<0.001).On the contrary,VEGF-C knockout obviously decreased the number of tube-like structures(P<0.001).Conclusions OSCC-associated macrophages can promote lymphangiogenesis,and VEGF-C secreted by the OSCC-associated macrophages may be an important role in that pathway.

关 键 词：口腔鳞癌 巨噬细胞 淋巴管生成 VEGF-C 

分 类 号：R739.8[医药卫生—肿瘤]