iTRAQ-based quantitative proteomics analysis of defense responses triggered by the pathogen Rhizoctonia solani infection in rice  

作　　者：FENG Zhi-ming GAO Peng ZHAO Jian-hua WANG Guang-da ZHANG Hui-min CAO Wen-lei XUE Xiang ZHANG Ya-fang MA Yu-yin HUA Rong CHEN Zong-xiang CHEN Xi-jun HU Ke-ming ZUO Shi-min 

机构地区：[1]Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding,Agricultural College,Yangzhou University,Yangzhou 225009,P.R.China [2]Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province,Yangzhou University,Yangzhou 225009,P.R.China [3]Joint International Research Laboratory of Agriculture and Agri-Product Safety,Ministry of Education of China/Institutes of Agricultural Science and Technology Development,Yangzhou University,Yangzhou 225009,P.R.China [4]Yangzhou Polytechnic College,Yangzhou 225000,P.R.China [5]Jiangsu Hongqi Seed Stock Co.,Ltd.,Taizhou 225311,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第1期139-152,共14页农业科学学报（英文版）

基　　金：financially supported by grants from the National Key Research and Development Program of China(2016YFD0100601);the National Natural Science Foundation of China(31701057 and 31672013);the Natural Science Foundation of Jiangsu Province,China(BK20170487);the Fok Ying Tung Education Foundation,China(151026);the China Postdoctoral Science Foundation(2017M620227);a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions,China(PAPD);the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(19KJD210001);2018 Annual Key Project of Scientific Research in Yangzhou Polytechnic College,China(2018ZR07)。

摘　　要：The soil-borne necrotrophic fungus Rhizoctonia solani is one of destructive fungi causing severe yield losses in various important crops. However, the host defense mechanisms against the invasion of this pathogen are poorly understood. In this study, we employed an i TRAQ-based quantitative proteomic approach to investigate host proteins responsive to R. solani using the resistant rice cultivar YSBR1. As a whole, we identified 319 differentially accumulated proteins(DAPs) after inoculation of rice plants with R. solani. Functional categorization analysis indicates that these DAPs cover a broad range of functions. Notably, a substantial portion of the DAPs are involved in cell redox homeostasis, carbohydrate metabolism, and phenylpropanoid biosynthesis, or belong to pathogenesis-related proteins, indicating that these processes/proteins play important roles in host defense against R. solani. Interestingly, all of the DAPs involved in photosynthesis and chlorophyll biosynthetic processes, and part of the DAPs involved in phenylpropanoid biosynthesis, show reduced accumulation after R. solani infection, suggesting that R. solani probably inhibits host photosynthetic system and phenylpropanoid biosynthesis to facilitate infection and colonization. In conclusion, our results provide both valuable resources and new insights into the molecular mechanisms underlying rice and R. solani interaction.

关 键 词：rice sheath blight Rhizoctonia solani PROTEOMIC defense response 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]