Genome-wide identification and analysis of the regulation wheat DnaJ family genes following wheat yellow mosaic virus infection  

作　　者：LIU Ting-ting XU Miao-ze GAO Shi-qi ZHANG Yang HU Yang JIN Peng CAI Lin-na CHENG Ye CHEN Jian-ping YANG Jian ZHONG Kai-li 

机构地区：[1]State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products/Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Rural Affairs and Zhejiang Province,Institute of Plant Virology,Ningbo University,Ningbo 315211,P.R.China [2]Institutes of Biomedical Sciences,Fudan University,Shanghai 200032,P.R.China [3]Institute of Forest Health,Zhejiang Academy of Forestry,Hangzhou 310021,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第1期153-169,共17页农业科学学报（英文版）

基　　金：supported by the National Key R&D Program of China(2018YFD0200507,2017YFD-0201701,and 2018YFD0200408);the National Natural Science Foundation of China(31901954);the Natural Science Foundation of Ningbo City,China(2019A610415 and 2019A610410);the National Key Project for Research on Transgenic Biology,China(2016ZX08002-001);the China Modern Agricultural Industry Technology System of MOF and MARA(CARS-03);the K.C.Wong Magna Funding in Ningbo University,China。

摘　　要：The co-chaperone DnaJ plays an important role in protein folding and regulation of various physiological activities, and participates in several pathological processes. DnaJ has been extensively studied in many species including humans,drosophila, mushrooms, tomatoes, and Arabidopsis. However, few studies have examined the role of DnaJ in wheat(Triticum aestivum), and the interaction mechanism between TaDnaJs and plant viruses. Here, we identified 236 TaDnaJs and performed a comprehensive genome-wide analysis of conserved domains, gene structure and protein motifs, chromosomal positions and duplication relationships, and cis-acting elements. We grouped these Ta Dna Js according to their domains, and randomly selected six genes from the groups for tissue-specific analysis, and expression profiles analysis under hormone stress, and 17 genes for plant virus infection stress. In qRT-PCR, we found that among the 17 TaDnaJ genes tested, 16 genes were up-regulated after wheat yellow mosaic virus(WYMV) infection, indicating that the TaDnaJ family is involved in plant defense response. Subsequent yeast two-hybrid assays verified the WYMV NIa, NIb and 7 KD proteins interacted with TaDJC(TraesCS7 A02 G506000), which had the most significant changes in gene expression levels after WYMV infection.Insights into the molecular mechanisms of Ta Dna J-mediated stress tolerance and sensitivity could inform different strategies designed to improve crop resistance to abiotic and biotic stress. This study provides a basis for future investigation of the TaDnaJ family and plant defense mechanisms.

关 键 词：TaDnaJ WYMV WHEAT GENOME-WIDE expression HORMONE biotic stress HSP40 

分 类 号：S435.121.4[农业科学—农业昆虫与害虫防治]