诺尔斯链霉菌固态发酵条件的优化研究  

Study on the Optimum of Solid Fermentation Parameters for Streptomyces Noursei

作　　者：谷军[1] 张晓彦[1] 于德水[1] Gu Jun;Zhang Xiaoyan;Yu Deshui(Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin 150010, China)

机构地区：[1]黑龙江省科学院微生物研究所,哈尔滨150010

出　　处：《黑龙江科学》2021年第24期44-47,共4页Heilongjiang Science

基　　金：黑龙江省科技厅重点专项“寒区规模化奶牛场粪污与秸秆综合利用制取生物天然气技术研究”(GA19B202)。

摘　　要：为了在诺尔斯链霉菌固体发酵生产过程中提高诺尔斯链霉菌分生孢子产量,选择不同的发酵原料并确定培养基的优化配比,优化固体发酵控制条件。结果表明,固体发酵过程中,玉米粉作用显著,可显著提高固体发酵诺尔斯链霉菌的分生孢子数,达到7.8×109 CFU/g。诺尔斯链霉菌的最优培养基为:豆饼粉6.28%、麸皮10.15%、硫酸铵0.2%、磷酸氢二钾0.1%,载体为玉米粉,原料含水量35%,发酵温度为30℃,发酵培养时间为9 d,可用于生产实践。In order to improve the production of streptomyces noursei spores number during streptomyces noursei solid state fermentation production process,different fermentation materials and the optimal ratio of culture medium were selected,and the control conditions of solid fermentation were optimize.During the fermentation process,corn flour can significant increase the number of streptomyces noursei spores number,reaching to 7.8×109 CFU/g.The best medium optimization for streptomyces noursei were 6.28%soybean cake flour,10.15%bran,0.2%ammonium sulfate,0.1%dipotassium hydrogen phosphate,corn meal as the carrier,35%water content of the raw material,30℃fermentation temperature,9 days fermentation time.It can be used in production practice.

关 键 词：诺尔斯链霉菌 固态发酵 正交设计 分生孢子数 

分 类 号：Q815[生物学—生物工程]