不同产地黑枸杞中总酚含量的比较研究  

A comparative study of total phenolic content in Lycium ruthenicum from different habitats

作　　者：王海燕 何文彬 李际红[3] 李玉琴 WANG Haiyan;HE Wenbin;LI Jihong;LI Yuqin*(Taian Institute for Food and Drug Control,Taian 271016,China;School of Pharmaceutical Sciences,Shandong First Medical University and Shandong Academy of Medical Science,Taian 271016,China;Forestry College,Shandong Agricultural University,Taian 271000,China)

机构地区：[1]泰安市食品药品检验检测研究院,山东泰安271016 [2]山东第一医科大学(山东省医学科学院)药学院,山东泰安271016 [3]山东农业大学林学院,山东泰安271000

出　　处：《中国药剂学杂志（网络版）》2022年第1期33-39,共7页Chinese Journal of Pharmaceutics：Online Edition

基　　金：山东省中医药科技发展计划(2019-0355)。

摘　　要：采用福林-酚比色法测定不同产地的黑枸杞中总酚的含量,比较不同产地黑枸杞中总酚含量的差异。酚类化合物中的酚羟基与福林酚试剂反应,产生蓝色复合物,以没食子酸为标准品,在760 nm处测定吸光度。没食子酸标准品在0.5μg/mL~2.5μg/mL范围内线性关系良好;青海柴达木产黑枸杞中中检测到总酚含量为17.40 mg/g;甘肃张掖产黑枸杞中总酚含量分别为15.02 mg/g;宁夏中宁产黑枸杞中总酚的含量分别为13.49 mg/g。不同产地的黑枸杞之间存在总酚含量上的差异,总酚含量趋势为青海柴达木>甘肃张掖>宁夏中宁。The total phenols in Lycium ruthenicum from different producing areas by Folin-Phenol colorimetry were determined to compare the difference of total phenolic contents in different species of black peony.The phenolic hydroxyl in the phenolic compound were reacted with the Folin-Phenol reagent to produce a blue complex,and the absorbance was measured at 760 nm using gallic acid as the standard.The linearity of gallic acid standard was good in the range of 0.5μg/mL~2.5μg/mL.The total phenolic contents were 17.40 mg/g,15.02 mg/g and 13.49 mg/g in Lycium ruthenicum from Qinghai Chaidamu,Gansu Zhangye and Ningxia Zhongning,respectively.There were differences in the contents of total phenols between Lycium ruthenicum from different habitats,and the trend of total phenol content was that Qinghai Chaidam>Gansu Zhangye>Ningxia Zhongning.

关 键 词：黑枸杞 不同产地 总酚 含量比较 

分 类 号：R94[医药卫生—药剂学] R917[医药卫生—药学]