Transcranial focused ultrasound stimulation reduces vasogenic edema after middle cerebral artery occlusion in mice  

作　　者：Li-Dong Deng Lin Qi Qian Suo Sheng-Ju Wu Muyassar Mamtilahun Ru-Bing Shi Ze Liu Jun-Feng Sun Yao-Hui Tang Zhi-Jun Zhang Guo-Yuan Yang Ji-Xian Wang 

机构地区：[1]Department of Rehabilitation Medicine,Ruijin Hospital,School of Medicine,and Med-X Research Institute and School of Biomedical Engineering,Shanghai Jiao Tong University,Shanghai,China [2]Department of Neurology,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,China

出　　处：《Neural Regeneration Research》2022年第9期2058-2063,共6页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,Nos.81802232(to JXW),81801170(to YHT),82071284(to YHT),2019YFA0112000(to YHT);the Scientific Research and Innovation Program of Shanghai Education Commission,No.2019-01-07-00-02-E00064(to GYY);Scientific and Technological Innovation Act Program of Shanghai Science and Technology Commission,No.20JC1411900(to GYY);Science and Technology Commission of Shanghai,No.19441907900(to JFS).

摘　　要：Blood-brain barrier(BBB)disruption underlies the vasogenic edema and neuronal cell death induced by acute ischemic stroke.Reducing this disruption has therapeutic potential.Transcranial focused ultrasound stimulation has shown neuromodulatory and neuroprotective effects in various brain diseases including ischemic stroke.Ultrasound stimulation can reduce inflammation and promote angiogenesis and neural circuit remodeling.However,its effect on the BBB in the acute phase of ischemic stroke is unknown.In this study of mice subjected to middle cerebral artery occlusion for 90 minutes,low-intensity low-frequency(0.5 MHz)transcranial focused ultrasound stimulation was applied 2,4,and 8 hours after occlusion.Ultrasound stimulation reduced edema volume,improved neurobehavioral outcomes,improved BBB integrity(enhanced tight junction protein ZO-1 expression and reduced IgG leakage),and reduced secretion of the inflammatory factors tumor necrosis factor-αand activation of matrix metalloproteinase-9 in the ischemic brain.Our results show that low-intensity ultrasound stimulation attenuated BBB disruption and edema formation,which suggests it may have therapeutic use in ischemic brain disease as a protector of BBB integrity.

关 键 词：blood-brain barrier brain edema cerebral blood flow ISCHEMIA matrix metalloproteinase-9 neurobehavioral outcomes tight junction transcranial ultrasound 

分 类 号：R743.3[医药卫生—神经病学与精神病学]