高脂饮食诱导C57BL/6J小鼠肥胖及减重手术模型的建立  

Establishment of high-fat diet-induced obesity and sleeve gastrectomy in C57BL/6J mice

作　　者：马媛媛 葛圣金 MA Yuan-yuan;GE Sheng-jin(Department of Anesthesia,Zhongshan Hospital,Fudan University,Shanghai 200032,China)

机构地区：[1]复旦大学附属中山医院麻醉科,上海200032

出　　处：《复旦学报（医学版）》2022年第1期88-93,共6页Fudan University Journal of Medical Sciences

基　　金：上海市自然科学基金(20ZR1410700)。

摘　　要：目的建立饮食诱导的肥胖小鼠模型,研究建立肥胖小鼠胃袖状切除术模型的可行性及有效性。方法将40只4周龄雄性SPF级C57BL/6J小鼠随机分为普通饮食组(n=20)和高脂饮食组(n=20),均喂养20周。20周后随机分为4个亚组:普通饮食假手术组,普通饮食手术组,高脂饮食假手术组,高脂饮食手术组。定期测量体重及空腹血糖,喂养20周后检测葡萄糖耐量、血脂水平及肝组织病理学。结果高脂饮食喂养4周后,高脂饮食组小鼠体重即开始显著高于普通饮食小鼠(P<0.05)。喂养20周后,与普通饮食小鼠相比,高脂饮食小鼠平均体重[(42.71±2.30)g vs.(32.23±1.73)g]、血糖[(8.82±0.51)mmol/L vs.(6.74±0.31)mmol/L]及血脂水平[血清总胆固醇:(4.40±0.57)mmol/L vs.(2.31±0.35)mmol/L;血清三酰甘油:(2.26±0.25)mmol/L vs.(1.11±0.24)mmol/L]均明显升高(P均<0.01)。高脂饮食及普通饮食假手术组小鼠存活率为100%,高脂饮食手术组存活率为70%(7/10),普通饮食手术组存活率为80%(8/10)。术后第1周,各组小鼠体重均显著下降(P<0.01);术后第4周,高脂饮食及普通饮食手术组小鼠较术前体重下降约20%;术后第3周,高脂饮食及普通饮食假手术组小鼠体重恢复至术前水平。结论高脂饮食喂养20周可诱导肥胖小鼠模型,进一步建立胃袖状切除术模型可行且有效。Objective To establish diet-induce obese model and explore the feasibility and effectiveness of sleeve gastrectomy(SG)model.Methods Forty 4-week-old male SPF C57BL/6J mice were randomly divided into two group:standard diet(SD)group(n=20),high-fat diet(HFD)group(n=20),and were fed for 20 weeks.After 20 weeks,they were randomly divided into 4 subgroups:SD+sham group,SD+SG group,HFD+sham group and HFD+SG group.Body weight and fasting blood glucose were measured,and glucose tolerance,blood lipid levels and histopathology were measured after 20 weeks.Results After 4 weeks,the average body weight in HFD group was significantly higher than those in SD group(P<0.05).After 20 weeks,the average body weight[(42.71±2.30)g vs.(32.23±1.73)g],blood glucose[(8.82±0.51)mmol/L vs.(6.74±0.31)mmol/L]and the serum lipid concentrations[total cholesterol:(4.40±0.57)mmol/L vs.(2.31±0.35)mmol/L;triacylglycerol:(2.26±0.25)mmol/L vs.(1.11±0.24)mmol/L]were significantly higher than those in SD group(all P<0.01).The survival rate in HFD+sham group and SD+sham group were 100%,in HFD+SG group it was 70%(7/10)and in SD+SG group it was 80%(8/10).The body weight in each group decreased significantly in postoperative one week(P<0.05);the mice lost about 20%of their preoperative weight in HFD+SG group and SD+SG group in the fourth week after surgery;the bodyweight recovered to the preoperative level in HFD+sham group and SD+sham group in the third week after surgery.Conclusion High-fat diet for 20 weeks could induce obese mice successfully,and sleeve gastrectomy model is effective and feasible.

关 键 词：肥胖 高脂饮食 胃袖状切除术 动物模型 

分 类 号：R614[医药卫生—麻醉学]