利用BSA-seq快速定位水稻抗褐飞虱QTL  

Rapid Mapping of Rice Brown Planthopper Resistance QTL by BSA-seq

作　　者：胡杰 何龙 张翔 岑浩伦 黄力坤 吴为人 Hu Jie;He Long;Zhang Xiang;Cen Haolun;Huang Likun;Wu Weiren(Key Laboratory of Genetics,Breeding and Multiple Utilization of Crops,Ministry of Education,Fujian Key Laboratory of Crop Breeding by Design,College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou,350002)

机构地区：[1]福建农林大学农学院,作物设计育种福建省重点实验室,教育部作物遗传育种与综合利用重点实验室,福州350002

出　　处：《分子植物育种》2021年第24期8184-8190,共7页Molecular Plant Breeding

基　　金：福建农林大学杰出青年基金项目(KXJQ18012);福建农林大学科技创新项目(KFA20006A)共同资助。

摘　　要：褐飞虱为害是造成水稻减产的一个重要因素。对水稻抗褐飞虱基因的发掘、鉴定和克隆是水稻功能基因组学的一个研究热点领域,也为抗虫育种提供了多样化的基因资源。本研究利用两个分别来自印度和孟加拉国的农家抗虫品系ARC14544和GUIAROI分别与感虫品种‘9311’和‘珍汕97’杂交,构建了两个F2:3群体,通过基于GBS简化基因组测序技术的混合分离分析法(BSA-seq)对抗褐飞虱QTL进行定位。两个群体同时在第4染色体20~22 Mb区间定位到的一个主效QTL qBPH4.5,与已克隆的褐飞虱抗性基因Bph6的位置相近。进一步利用此区间的分子标记RM5757,证实该QTL在两个群体中是真实存在的。本研究展示了BSA-seq法的有效性,为水稻褐飞虱抗性的分子标记辅助育种提供了基础。The damage of brown planthopper is an important factor causing reduction of rice yield.Exploring,identifying and cloning BPH resistance genes is a hotspot of functional genomic study in rice,and can also provide diverse gene resources for BPH-resistance breeding.In this study,two F2:3 populations were developed from the crosses between’9311’and ARC14544(a resistant cultivar from India)and between’Zhenshan97’and GUIAROI(a resistant cultivar from Bangladesh)to map QTLs for BPH resistance using the method of genotyping by sequencing(GBS)-based bulked segregant analysis(BSA-seq).A major QTL,qBPH4.5,was mapped in the region20~22 Mb on chromosome 4 in both populations,very close to the cloned gene Bph6.We further used a molecular marker RM5757 in this interval to analyze the effect of qBPH4.5,confirming that the QTL really existed in the two populations.This study demonstrated the efficiency of BSA-seq and provided a basis for the molecular marker-assisted breeding of BPH resistance in rice.

关 键 词：水稻 抗褐飞虱基因 QTL定位 BSA-seq 

分 类 号：S435.112.3[农业科学—农业昆虫与害虫防治]