HS-SPME-GC-MS联用测定浙江省7种樱属植物花的挥发性成分  

Determination of Volatile Components from Flowers of 7 Species of Cerasus in Zhejiang Province by HS-SPME-GC-MS

作　　者：付涛[1] 王志龙[1] 李文[1] 陈际伸[1] Fu Tao;Wang Zhilong;Li Wen;Chen Jishen(Ningbo Key Laboratory of Landscape Plant Development,Ningbo City College of Vocational Technology,Ningbo,315100)

机构地区：[1]宁波城市职业技术学院浙江园林绿化技术协同创新中心,宁波315100

出　　处：《分子植物育种》2021年第24期8287-8296,共10页Molecular Plant Breeding

基　　金：宁波城市职业技术学院校内科研青年专项课题(ZZX18122,ZZX18123,ZZX18128);宁波市自然科学基金项目(ZZT18104)共同资助。

摘　　要：比较浙江省樱属7种植物花中挥发性成分组成的差异,并探讨7种植物之间的亲缘关系,为中国樱属植物的资源利用提供理论依据。采用顶空固相微萃取(HS-SPME)方法处理样品,并优化前处理条件,结合气相色谱-质谱联用(GC-MS)技术测定樱属7种植物花中的挥发性成分,比较7种植物花中的成分差异。然后应用SPSS 22软件进行主成分分析(PCA),结果显示,评分图直观地反映了7种樱属植物之间的关系。通过对样品处理条件的优化,HS-SPME最佳的萃取条件为1.0 g的样品用量、70℃的吸附水浴温度以及40 min的吸附时间。通过GC-MS测定并定性分析,从浙江省7种樱属植物花中共鉴定出42种挥发性成分,其中野生早樱鉴定出的物质最多,有21种,沼生矮樱鉴定出的物质最少,仅有7种,主要为醛类、醇类和酯类物质,有机酸类、酮类和烃类等物质含量较少;其中醛类含量均占88%以上,醛类中又以苯甲醛为最高,含量均达80%以上,该成分不仅含量高,而且香气阈值较低,对樱属植物特殊气味起到至关重要的作用。主成分分析表明,除沼生矮樱外,其余樱属植物聚在一起,支持形态分类将其归入矮生樱亚属的结果。本研究结果可为中国樱属植物资源的合理开发与利用奠定理论基础。The differences of volatile components in the flowers of seven species of Cerasus in Zhejiang Province were compared,and the genetic relationships among the seven species were discussed to provide theoretical basis for the utilization of Cerasus resources in China.The samples were treated by HS-SPME and the pretreatment conditions were optimized.Combined with GC-MS technology,the volatile components from flowers of 7 species of Cerasus were determined,and the difference of them was compared.Then,SPSS 22 software was applied to conduct the PCA of the 7 species of Cerasus flowers.According to the score diagrams,the relationship among the7 species of Cerasus was visually presented.By optimizing treatment conditions of the samples,the best extraction conditions of SPME were 1.0 g of sample weight,70℃of heating temperature,40 min of adsorption time.GC-MS qualitative analysis,42 volatile components were identified from the 7 species of Cerasus.Among them,there are21 kinds of substances identified by Cerasus subhirtella var.ascendens and 7 kinds by Cerasus jingningensis,it is mainly aldehydes,alcohols and esters,with less organic acids,ketones and hydrocarbons,among which aldehydes account for more than 88%,and benzaldehyde is the highest among aldehydes,with the content of more than 80%,this component is not only high in content,but also low in aroma threshold,which plays an important role in the special smell of Cerasus plants.The results of PCA showed that other Cerasus plants gathered together,except for the macrophytes,which supported the morphological classification to classify them into the Cerasus Subgen.Microcerasus.The results of this study can provide a theoretical basis for the development and utilization of Cerasus resources in China.

关 键 词：樱属 气相色谱-质谱法(GC-MS) 挥发性成分 主成分分析 

分 类 号：S685[农业科学—观赏园艺]