非小细胞肺癌组织miR-139-5p和CTNNB1蛋白表达与放射敏感性的关系  

The relationship between the expression of miR-139-5p and CTNNB1 protein and radiosensitivity in non-small cell lung cancer

作　　者：张静[1] 刘爽 王洁 姚淑晖 宋晶晶[1] 熊伟[1] 黄晓智[2] Zhang Jing;Liu Shuang;Wang Jie;Yao Shuhui;Song Jingjing;Xiong Wei;Huang Xiaozhi(The Seventh Department of Radiology and Chemistry,Tangshan People's Hospital,Hebei Province,Tangshan 063000,China;不详)

机构地区：[1]河北省唐山市人民医院放化七科,063000 [2]河北省唐山市人民医院肿瘤内科,063000

出　　处：《疑难病杂志》2022年第1期1-6,共6页Chinese Journal of Difficult and Complicated Cases

基　　金：河北省医学科学研究重点课题计划项目(20181233)。

摘　　要：目的探讨非小细胞肺癌组织中微小RNA-139-5p(miR-139-5p)、钙黏蛋白相关蛋白(CTNNB1)表达与患者放射敏感性的关系。方法选取2017年2月—2021年5月于唐山市人民医院放化七科和肿瘤内科就诊的非小细胞肺癌患者癌组织156例为研究对象(研究组);另选取非小细胞肺癌患者86例癌旁正常肺组织作为对照组;根据放射治疗敏感性评定标准,将研究组患者分为进展亚组(n=28)、稳定亚组(n=42)、缓解亚组(n=86)。采用实时荧光定量PCR和免疫组织化学法分别检测肺癌组织、癌旁正常肺组织miR-139-5p、CTNNB1蛋白表达;分析非小细胞肺癌患者癌组织miR-139-5p、CTNNB1蛋白表达与临床病理特征及放射敏感性的关系;Spearman法分析非小细胞肺癌患者癌组织miR-139-5p表达水平与CTNNB1蛋白阳性表达的相关性;Logistic回归分析影响非小细胞肺癌患者放射敏感性的因素。结果研究组癌组织miR-139-5p表达水平低于对照组(t/P=15.204/0.000),CTNNB1蛋白阳性表达率高于对照组(χ^(2)/P=59.206/0.000);miR-139-5p低表达患者中有淋巴结转移、组织分化程度低、TNM分期Ⅳ期患者比例高于miR-139-5p高表达患者(χ^(2)/P=16.006/0.000、24.669/0.000、22.213/0.000);CTNNB1蛋白阴性表达患者中有淋巴结转移、组织分化程度低、TNM分期Ⅳ期患者比例低于CTNNB1蛋白阳性表达患者(χ^(2)/P=21.922/0.000、22.959/0.000、30.108/0.000);非小细胞肺癌患者癌组织miR-139-5p表达水平与CTNNB1蛋白阳性表达呈负相关(r/P=-0.503/0.000);缓解亚组、稳定亚组、进展亚组miR-139-5p低表达率及CTNNB1蛋白阳性表达率依次升高(χ^(2)/P=25.762/0.000、18.721/0.000);CTNNB1蛋白阳性表达、TNM分期高是影响放射敏感性的独立危险因素[OR(95%CI)=2.872(1.546~5.335)、2.732(1.459~5.115)],miR-139-5p高表达是影响放射敏感性的保护因素[OR(95%CI)=0.845(0.750~0.952)]。结论非小细胞肺癌组织中miR-139-5p表达下调、CTNNB1蛋白阳性表达上调,Objective To explore the relationship between the expression of microRNA1395p(miR-139-5p)and cadherin-related protein(CTNNB1)in non-small cell lung cancer tissues and the radiosensitivity of patients.Methods One hundred and fifty-six patients with non-small cell lung cancer who were treated in the Seventh Department of Radiochemistry and Oncology of Tangshan People's Hospital from February 2017 to May 2021 were selected as the research objects(research group).In addition,86 patients with non-small cell lung cancer patients with normal lung tissue adjacent to cancer were selected as the control group.According to the radiotherapy sensitivity evaluation criteria,patients in the study group were divided into progression subgroup(n=28),stable subgroup(n=42),and remission subgroup(n=86).Real-time fluorescence quantitative PCR and immunohistochemistry were used to detect the expression of miR-139-5p and CTNNB1 protein in lung cancer tissue and normal lung tissue adjacent to cancer.The relationship between the expression of miR-139-5p and CTNNB1 protein in cancer tissues of patients with non-small cell lung cancer and the clinicopathological characteristics and radiosensitivity were analyzed.Spearman method was used to analyze the correlation between the expression of miR-139-5p and the positive expression of CTNNB1 protein in cancer tissues of patients with non-small cell lung cancer.Logistic regression analysis of factors affecting radiosensitivity of patients with non-small cell lung cancer.Results The expression level of miR-139-5p in cancer tissues of the study group was lower than that of the control group(χ^(2)/P=15.204/0.000),and the positive expression rate of CTNNB1 protein was higher than that of the control group(χ^(2)/P=59.206/0.000).The proportion of patients with low expression of miR-139-5p with lymph node metastasis,low tissue differentiation,and TNM stage IV was higher than that of patients with high expression of miR-139-5p(χ^(2)/P=16.006/0.000,24.669/0.000,22.213/0.000).The proportion of patients

关 键 词：非小细胞肺癌 微小RNA-139-5p 钙黏蛋白相关蛋白 放射敏感性 

分 类 号：R734.2[医药卫生—肿瘤]