Distinctive quality control method for solid-state fermented Isaria cicadae from strain Ic-17-7 and application in a rat model of type 2 diabetes  

作　　者：SUN Chang-Sheng WANG Yu-Qin ZHANG Zhong-Liang LIOU Yuligh ZHOU Hong-Hao 

机构地区：[1]Department of Clinical Pharmacology,Xiangya Hospital,Central South University,Changsha 410008,China [2]Institute of Clinical Pharmacology,Central South University,Hunan Key Laboratory of Pharmacogenetics,Changsha 410078,China [3]Engineering Research Center of Applied Technology of Pharmacogenomics,Ministry of Education,Changsha 410078,China [4]National Clinical Research Center for Geriatric Disorders,Changsha 410008,China [5]China Zhejiang Bioasia Life Science Institute,Pinghu 314200,China

出　　处：《Chinese Journal of Natural Medicines》2021年第12期921-929,共9页中国天然药物（英文版）

基　　金：supported by China Spark Program (No.2015GA700011)。

摘　　要：This work was aimed to establish a quality control method for evaluating the effects on glucose and lipids of the fruiting body of Isaria cicadae Miquel from strain Ic-17-7(Ic-17-7fb) using a rat model of type 2 diabetes(T2DM). Random amplified polymorphic DNA, sequence-characterized amplified region, and high-performance liquid chromatography(HPLC) were used for the quality control of Ic-17-7fb. The pharmacological effects on streptozocin(STZ)-induced high fat diet(HFD)-fed Albino Wistar rats were evaluated. The rats underwent the following treatments: control, metformin, Ic-17-7fb(0.166 and 0.5 g·kg;) or without treatment. The fasting blood glucose(FBG), blood glucose, total cholesterol(TC), triglyceride(TG), high-density lipoprotein(HDL-c), and low-density lipoprotein(LDL-c) were measured. Ic-17-7fb amplified a single specific band by S11-2-F3 and S11-2-R3 primers. An HPLC-based quality and quantity method was established for industrial application. The contents of adenosine and N;-(2-hydroxyethyl) adenosine(HEA) of the cultivated Ic-17-7fb were analyzed. All of the validation lots of cultured Ic-17-7fb passed the quantity control of the training set(0.90 mg·g;of adenosine and 0.89 mg·g;of HEA). After two weeks of administration, the average FBG was 4.89 ± 0.42(control), 26.10 ± 5.77(model), 23.63 ± 6.15(metformin), 17.96 ± 9.36(Ic-17-7fb for 0.166 g·kg;), and 19.69 ±8.71 mmol·L;(Ic-17-7fb for 0.5 g·kg;). The FBG of Ic-17-7fb(0.166 g·kg;) treatment significantly reduced by 31.19%, compared with the model after two weeks of administration(P < 0.01). Metformin, Ic-17-7fb(0.166 g·kg;), and Ic-17-7fb(0.5 g·kg;) reduced TC, TG, HDL-c, and LDL-c compared with the T2DM model treatment at the 6 th week of treatment(P < 0.05). This study established the first quality standard for Ic-17-7fb, which can be effectively applied in the treatment of T2DM. The reliable quality control method and pharmacological effect will broaden its application space.

关 键 词：Isaria cicadae Quality control ADENOSINE HEA Type 2 diabetes model Blood glucose and lipid 

分 类 号：R284[医药卫生—中药学] R285.5[医药卫生—中医学]