核桃花化学成分的研究  

Chemical constituents from flowers of Juglans regia

作　　者：李倩 LI Qian(Henan Province Engineering Research Center for Food Safety Control of Processing and Circulation,College of Food Science and Technology,Henan Agricultural University,Zhengzhou 450002,China)

机构地区：[1]河南农业大学食品科学技术学院,河南省食品加工与流通安全控制工程技术研究中心,河南郑州450002

出　　处：《中草药》2021年第24期7413-7419,共7页Chinese Traditional and Herbal Drugs

基　　金：河南农业大学科技创新基金资助项目(KJCX2021A19)。

摘　　要：目的研究核桃Juglans regia花的化学成分。方法利用正和反相硅胶、Sephadex LH-20及制备液相等柱色谱方法进行分离纯化,根据谱学数据及理化性质鉴定化合物结构,并采用MTT法检测化合物的体外细胞毒活性。结果从核桃花95%乙醇水提取物中分离得到12个黄酮类化合物和7个生物碱类化合物,分别鉴定为金合欢素(1)、柚皮素(2)、5,7-二羟基-6,8,4′-三甲氧基黄酮(3)、木犀草素(4)、草质素(5)、白杨素(6)、异野樱素(7)、3,5-二羟基-4′,7-二甲氧基黄酮醇(8)、槲皮苷(9)、异槲皮苷(10)、山柰酚(11)、槲皮素(12)、2-(4-羟基苯乙基)异吲哚啉-1,3-二酮(13)、吲哚-3-甲酸乙酯(14)、1,2,3,4-四氢去甲哈尔满-1-酮(15)、3-醛基吲哚(16)、马齿苋酰胺E(17)、青兰素C(18)、N-苯甲酰-L-苯丙氨酸(19)。结论除化合物4和9~12外,其余化合物均为首次从胡桃属中分离得到,其中化合物13为1个新的天然产物。化合物2、3和5具有抑制人结肠癌HCT-116细胞、人肝癌HepG2细胞、人胃癌BGC-823细胞、人肺支气管癌NCI-H1650细胞、人卵巢癌A2780细胞的增殖作用。Objective To investigate the chemical constituents from the flowers of Juglans regia. Methods The compounds were isolated and purified by various column chromatographies, and their structures were identified by physiochemical properties and spectroscopic data. MTT assay was used to detect the cytotoxic activity of the compounds in vitro. Results Ninteen compounds were isolated from the EtOH extract of the flowers of J. regia, which were identified as acacetin(1), naringenin(2),5,7-dihydroxy-6,8,4′-dimethoxyflavone(3), luteolin(4), herbacetin(5), chrysin(6), isosakuranetin(7), 3,5-dihydroxy-4′,7-dimethoxyflavone(8), quercitrin(9), isoquercitrin(10), kaempferol(11), quercetin(12), 2-(4-hydroxyphenethyl)isoindoline-1,3-dione(13), 1 H-indole-3-carboxylic acid-ethyl ester(14), 1,2,3,4-tetrahydronorharman-1-one(15), 1 H-indole-3-carbaldehyde(16), oleracein E(17), dracocephin C(18), N-benzoyl-L-phenylalanine(19). Conclusion All compounds were isolated from this genus for the first time except for compounds 4, 9—12. Compounds 2, 3 and 5 exhibited significant in vitro growth inhibitory activity against the HCT-116, HepG2, BGC-823, NCI-H1650, and A2780 cancer cell lines.

关 键 词：核桃花 黄酮 生物碱 抗肿瘤 2-(4-羟基苯乙基)异吲哚啉-1 3-二酮 柚皮素 5 7-二羟基-6 8 4′-三甲氧基黄酮 草质素 

分 类 号：R284.1[医药卫生—中药学]