TRP14促进非小细胞肺癌细胞增殖移动的作用机制研究  

Potential Mechanism of TRP14 Promotes the Cells Proliferation and Migration of Non-Small Cell Lung Cancer

作　　者：闫红江[1] 任红欣 高伟年[1] 马晓钰[1] 石彦涛[1] 张合林[1] YAN Hong-jiang;REN Hong-xin;GAO Wei-nian;MA Xiao-yu;SHI Yan-tao;ZHANG He-lin(Department of Thoracic Surgery,The Second Hospital of Hebei Medical University,Shijiazhuang 050000,China)

机构地区：[1]河北医科大学第二医院胸外科,石家庄050000

出　　处：《循证医学》2021年第5期294-300,共7页The Journal of Evidence-Based Medicine

基　　金：河北省医学科学研究课题(20210982)。

摘　　要：目的探究硫氧还蛋白相关蛋白14(thioredoxin-related protein 14 kDa,TRP14)在非小细胞肺癌(non-small cell lung cancer,NSCLC)细胞增殖移动过程中的功能及其作用机制。方法利用肿瘤基因图谱(The Cancer Genome Atlas,TCGA)数据研究并分析TRP14在NSCLC组织中的表达水平,定量聚合酶链反应(quantitative polymerase chain reaction,qPCR)检测TRP14在正常肺上皮细胞和NSCLC细胞及组织中的表达;在H1299和H1975细胞系中用克隆形成实验检测TRP14基因敲减后对NSCLC细胞增殖的影响,探索TRP14在NSCLC细胞增殖中的作用,并用CCK8细胞增殖转移、细胞凋亡实验进一步验证;将TRP14干扰后,用western blot实验检测下游通路基因以分析TRP14基因可能的作用机制。结果与癌旁组织相比,TRP14基因在肺癌组织表达显著升高,且TRP14敲减能抑制细胞增殖和转移、促进细胞凋亡;进一步发现TRP14可能通过调控细胞自噬发挥作用。结论TRP14基因可能在NSCLC的细胞增殖移动中起到重要的作用,该作用可能通过影响细胞自噬机制进行,TRP14基因可能成为NSCLC的一个新的潜在的分子诊断指标及靶点。Objective To explore the function and mechanism of thioredoxin-related protein 14 kDa(TRP14)in the cells proliferation and migration of non-small cell lung cancer(NSCLC).Methods Analysis the expression level of TRP14 in NSCLC within The Cancer Genome Atlas(TCGA)data.The expression of TRP14 in lung epithelial cells and NSCLC cells were detected by quantitative polymerase chain reaction(qPCR).Knockdown the TRP14 gene to detect the proliferation of NSCLC cells H1299 and H1975 by CCK8 assay and clone formation assay,and detect the migration and apoptosis by transwell and flow cytometry.After TRP14 was interfered,the downstream pathway genes were detected by western blot to analyze the possible mechanism of TRP14 gene.Results TRP14 expression was significantly increased in NSCLC tissues compared with adjacent tissues,and TRP14 knockdown inhibited cell proliferation and migration,and promoted cell apoptosis.Moreover,TRP14 may play a role by regulating autophagy.Conclusions TRP14 gene played an important role in the cells proliferation and migration of NSCLC by influencing the mechanism of autophagy.TRP14 gene was a new potential molecular diagnostic index and target for NSCLC.

关 键 词：TRP14基因 非小细胞肺癌 自噬 

分 类 号：R734.2[医药卫生—肿瘤]