虹鳟hif-1a基因家族的拷贝数变异及分歧进化  

Copy Number Variation and Divergence of hif-1a Gene Family in Oncorhynchus mykiss

作　　者：王宪宗 宋晶[1] 杨春娟 WANG Xianzong;SONG Jing;YANG Chunjuan(College of Animal Science,Shanxi Agricultural University,Taigu 030801,China;Station for Popularizing Aquatic Technology of Shanxi Province,Taiyuan 030002,China)

机构地区：[1]山西农业大学动物科学学院,山西太谷030801 [2]山西省水产技术推广站,山西太原030002

出　　处：《河南农业科学》2021年第11期153-161,共9页Journal of Henan Agricultural Sciences

基　　金：山西省高等学校科技创新项目(2020L0158);山西农业大学科技创新项目(2017YJ05)。

摘　　要：为深入阐明虹鳟不耐低氧的分子机制,采用BLAST搜索、重构最大似然树来确定其hif-1a家族的拷贝数,并对这些拷贝的组织表达谱、共表达基因及其所富集的GO条目、编码蛋白质的保守结构域分布及部分结构域的三维结构进行比较。结果显示,虹鳟的hif-1a有2个拷贝,与它们直系同源的斑马鱼基因均是hif-1aa。2个hif-1a拷贝的组织表达谱存在显著的分化,它们的共表达基因数量及所富集的与血管生成、细胞增殖、物质运输和代谢相关的GO条目数量也存在较大差异;它们所编码的蛋白质均拥有5个保守结构域,C-TAD结构域三维结构均存在结构创新,但bHLH结构域的三维结构存在较大差异。综上,虹鳟在较近的全基因组重复事件之前丢失了hif-1ab,现有的2个hif-1aa拷贝在朝亚功能化方向快速进化,某些地理种群中可能已经存在有利于提高耐低氧性能的基因型。To further elucidate the molecular mechanism of hypoxia intolerance of Oncorhynchus mykiss,BLAST search and reconstruction of maximum likelihood tree were conducted to determine the copy number of hif-1 a family. Tissue expression profiles,co-expressed genes and their enriched GO terms,distribution of conserved domains and three-dimensional structures of partial domains were compared between these copies. The results showed that there were two copies of hif-1 a in O. mykiss,the orthologs of which in Danio rerio were all hif-1 aa. The two copies varied significantly in tissue expression profiles.Their co-expressed genes and GO terms related to angiogenesis,cell proliferation,material transport and metabolism enriched by these co-expressed genes also varied greatly in number. The proteins encoded by them both contained five conserved domains,among which the two C-TAD domains both contained structural innovation,while the two bHLH domains varied greatly in three-dimensional structure.Collectively,O. mykiss lost hif-1 ab before the recent whole genome duplication event,while the two existing hif-1 aa copies have been rapidly evolving towards subfunctionalization. Some geographical populations might already have genotypes that are helpful to improve hypoxia tolerance.

关 键 词：虹鳟 hif-1a基因家族 低氧 全基因组重复 共表达基因 亚功能化 

分 类 号：S917.4[农业科学—水产科学]