液相色谱-光谱图法定性确证食品中苯甲酸、山梨酸  

Qualitative identify of benzoic acid and sorbic acid in food by using liquid chromatography-spectrum

作　　者：马长海 于明明 闫吉昌 辛若竹 MA Changhai;YU Mingming;YAN Jichang;XIN Ruozhu(Baicheng Quality Supervision and Inspection Station of Product,Baicheng 137000,China;Meihekou Center for Food and Drug Control,Meihekou 135000,China)

机构地区：[1]白城市产品质量检验所,吉林白城137000 [2]梅河口市食品药品检验检测中心,吉林梅河口135000

出　　处：《化学分析计量》2022年第1期45-48,共4页Chemical Analysis And Meterage

摘　　要：建立液相色谱-光谱图法定性确证食品中的苯甲酸、山梨酸。样品经提取后,以Agilent Poroshell 120 EC-C_(18)色谱柱分离,2 mmol/L甲酸与20 mmol/L乙酸铵混合溶液-甲醇(体积比为95∶5)为流动相洗脱,于230 nm波长检测,同时利用二极管阵列检测器全光谱扫描功能(190~400 nm,步进值2.0 nm)分别采集标准溶液和样品溶液的光谱图。以保留时间定性,通过比较标准溶液中苯甲酸、山梨酸的光谱图与样品溶液中可疑色谱峰光谱图的重合性,进一步定性确证样品中可疑色谱峰是否属于苯甲酸、山梨酸。该方法采用保留时间和光谱图信息两方面进行综合定性分析,可有效避免检测工作中因定性失误而出现假阳性样品。Benzoic acid and sorbic acid in food were qualitatively identified by using liquid chromatography-spectrum.After the sample was extracted,Agilent Poroshell 120 EC-C_(18) chromatography column was used for separation.The mixed solution of 2 mmol/L formic acids with 20 mmol/L ammonium acetate-methanol(95∶5)was used as the mobile phase for elution.The detection wavelength was 230 nm.Meanwhile,the spectrogram information of the standard solution and test sample solution were collected using the full-spectrum scan function of the DAD detector(190-400 nm,with step size of 2.0 nm).Besides qualitative identifying sample benzoic acid,sorbic acid by using chromatographic retention time,further confirmation was done by comparison of spectrum peak between the standard solution and test sample.False-positive error could be avoided by combing chromatographic retention time and spectrogram information.

关 键 词：液相色谱法 二极管阵列检测器 光谱图 定性确证 苯甲酸 山梨酸 假阳性 

分 类 号：O657.7[理学—分析化学]