茉莉酸对马铃薯苗期低温胁迫耐性的影响  

Effects of Jasmonic Acid on Cold Stress Tolerance of Potato at Seedling Stage

作　　者：刘计涛 王梦诗 索海翠[1] 罗焕明[1] 王丽[1] 李成晨 单建伟 廖永珊 李小波[1] LIU Jitao;WANG Mengshi;SUO Haicui;LUO Huanming;WANG Li;LI Chengchen;SHAN Jianwei;LIAO Yongshan;LI Xiaobo(Crops Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Crops Genetics&Improvement,Guangzhou 510640,China)

机构地区：[1]广东省农业科学院作物研究所/广东省农作物遗传改良重点实验室,广东广州510640

出　　处：《广东农业科学》2021年第12期129-137,共9页Guangdong Agricultural Sciences

基　　金：广东省农业科学院创新基金(202109);广东省自然科学基金(2021A1515010778);广州市科技计划项目(202102020845);广东省现代农业产业技术体系创新团队项目(2021KJ111)。

摘　　要：【目的】研究不同茉莉酸(JA)施用浓度对马铃薯叶片活性氧(ROS)含量及其清除酶活性以及抗寒关键基因CBF表达的影响,初步确定提升马铃薯抗寒性的最适施用浓度,为马铃薯产业的发展提供一定的理论基础。【方法】以马铃薯品种费乌瑞它为研究对象,采用叶面喷施JA的方法,探究正常和低温胁迫条件下,植株叶片的活性氧含量,活性氧清除酶过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶SOD活性,以及抗寒关键基因CBF的表达。【结果】随着JA浓度的增加,马铃薯苗期叶片的活性氧含量逐渐降低,低温胁迫后活性氧含量能维持在较低水平,适宜浓度范围为100~400μmol/L,以200μmol/L JA处理效果最好,ROS含量降低47.6%。JA能够提升活性氧清除酶CAT和POD活性,且随着JA浓度增加效果越显著,低温胁迫后200μmol/L JA处理分别显著提升1.30、2.53倍。正常条件下,JA处理抑制SOD活性最低达70.7%;低温胁迫条件下,JA又可能通过其他途径提升SOD活性,最高达2.11倍。外源JA能诱导CBF1-3基因的表达,而低温胁迫能促进内源JA的合成,诱导CBF1-3基因的表达,提升马铃薯的抗寒性。【结论】马铃薯苗期的抗寒性与外源施加JA的浓度具有明显的相关性,适宜的JA浓度能够促进抗寒关键基因CBF1-3的表达,显著提升CAT、POD、SOD的活性,抑制低温胁胁迫诱导的活性氧生成,提高马铃薯抗寒性。适宜施用JA浓度为100~400μmol/L,以200μmol/L效果最佳。【Objective】The research was carried out to explore the effects of different jasmonic acid(JA)concentrations on the content of reactive oxygen species(ROS)and its scavenging enzyme activity as well as the expression of key cold resistance gene CBF of potato,and to identify the optimal application concentration of JA,which contributed to enhance cold tolerance of potato,with a view to providing a theoretical basis for the development of potato industry.【Method】Taking the potato variety Favorita as the research object,the method of spraying JA on potato leaves was used to explore the content of ROS,the activities of catalase(CAT),peroxidase(POD)and superoxide dismutase SOD,and the expression of CBF genes of potato leaves under the normal and low temperature conditions.【Result】As the increase of JA concentration,the content of ROS of potato leaves at seedling stage decreased gradually,and the content of ROS could be maintained at a low level upon cold stress.The appropriate concentration range was 100μmol/L to 400μmol/L,among which 200μmol/L had the most significant effect,and the ROS content was reduced by 47.6%compared with control.JA could enhance the activities of ROS scavenging enzymes-CAT and POD,and the effect became more significant with the increase of JA concentration.After cold stress,200μmol/L JA treatment could significantly increase the activities of CAT and POD by 1.30 and 2.53 times,respectively.However,under th e normal condition,JA treatment inhibited SOD activity to a minimum of 70.7%,while JA may increase SOD activity to a maximum of 2.11 times through other ways under the condition of cold stress.Exogenous JA could induce the expression of StCBF1-3,and cold stress could promote the synthesis of endogenous JA which might induce the expression of StCBF1-3 and improve the cold tolerance of potato.【Conclusion】The cold tolerance of potato is significantly correlated with the concentration of exogenous JA.The appropriate concentration can promote the expression of key gene StCBF1-3

关 键 词：马铃薯 茉莉酸 低温胁迫 活性氧 CBF基因 

分 类 号：S532.032[农业科学—作物学]