不同频率电针刺激对脑出血大鼠血肿脑组织脑红蛋白及NLRP3信号通路的影响  

Effects of different frequencies of electric acupuncture stimulation on neuroglobin and NLRP3 signaling pathways in hematoma brain tissue in rats with cerebral hemorrhage

作　　者：许文婷 薛玉满 王策[3] 孔莹 XU Wenting;XUE Yuman;WANG Ce;KONG Ying(Heilongjiang University of Traditional Chinese Medicine, Harbin 150001, Heilongjiang, China;The Second Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, Harbin 150001, Heilongjiang;Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 611130, Sichuan, China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150001 [2]黑龙江中医药大学附属第二医院,黑龙江哈尔滨150001 [3]成都中医药大学附属医院,四川成都611130

出　　处：《现代中西医结合杂志》2022年第1期10-15,共6页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金资助项目(81904305);黑龙江省博士后专项资助项目(LBH-Z12249);黑龙江中医药大学科研基金项目(2019MS24);黑龙江中医药大学博士创新基金项目(2017BS05)。

摘　　要：目的研究不同频率电针刺激对脑出血大鼠血肿脑组织中脑红蛋白(Ngb)及NLRP3信号通路相关指标的影响。方法将90只健康Wistar大鼠随机分为正常组、假手术组、模型组、电针1组、电针2组、电针3组,每组15只。模型组和电针各组以大鼠尾状核自体动脉血注入法复制脑出血模型。大鼠造模苏醒后,电针各组选取百会、水沟、双侧内关穴给予电针刺激治疗,电针1组、电针2组和电针3组分别设定电针频率为2 Hz、15 Hz和50 Hz,每日1次,共连续治疗10 d;假手术组采用套迭式顿头安慰针干预,但不刺入皮肤。采用Bederson评分标准对各组大鼠进行神经行为学评分,HE染色观察脑组织形态,Western blot法检测血肿脑组织中Ngb表达情况,ELISA法检测血肿脑组织中NLRP3、Caspase-1、白细胞介素-1β(IL-1β)、白细胞介素-18(IL-18)含量。结果实验24 h、48 h、5 d和10 d时,电针各组的神经行为学评分均显著低于模型组(P均<0.05),且随着时间延长和电针频率的增加,评分呈明显下降趋势;电针3组实验5 d和10 d时的神经行为学评分均显著低于电针1组(P均<0.05)。HE染色显示,模型组大鼠脑出血区域细胞排列不规则,可见大量变性坏死的神经细胞,细胞间隙变大,胞浆减少,多数可见核固缩;电针各组大鼠脑出血区域细胞形态均较模型组改善,且电针3组改善最为明显。电针各组血肿脑组织中Ngb表达量均明显高于模型组(P均<0.05),其中电针2组和电针3组均明显高于电针1组(P均<0.05);电针各组血肿脑组织中NLRP3、Caspase-1、IL-1β、IL-18含量均明显低于模型组(P均<0.05),且随着电针频率的增加呈现逐渐下降趋势。结论不同频率电针可影响脑出血大鼠的治疗效果,其中50 Hz高频率电针刺激较2 Hz、15 Hz低频率电针刺激的作用更佳,该治疗作用可能是通过调控血肿脑组织Ngb表达及NLRP3信号通路实现的。Objective It is to study the effects of electroacupuncture at different frequencies on neuroglobin(Ngb)and NLRP3 signaling pathway related indicators in hematoma brain tissue of rats with intracerebral hemorrhage.Methods Ninety rats were randomly divided into six groups:normal group,sham operation group,model group,electroacupuncture group I,electroacupuncture group II and electroacupuncture group III,each group had 15 rats.The cerebral hemorrhage models were replicated by autologous arterial blood infusion into the caudate nucleus of rats in the model group and the electroacupuncture group.After the rats were awakened from the modeling,the ones in each electroacupuncture group were treated with selected electroacupuncture stimulation at Baihui,Shuigou,and bilateral Neiguan.The electroacupuncture frequency was respectively set for 2 Hz,15 Hz,and 50 Hz in group I,group II and group III,once a day,totally continuously treated for 10 days;the rats in the sham operation group were treated with telescopic relief needle,but the skin was not penetrated.The scores of the neurobehavior of rats in each group were evaluated by Bederson scoring standard,the brain tissue morphology was observed by HE staining,the expression of Ngb in hematoma brain tissue was detected by Western blot,and the contents of NLRP3,Caspase-1 and interleukin-1β(IL-1β),interleukin-18(IL-18)in hematoma brain tissue were detected by ELISA method.Results At 24h,48h,5d and 10d in the study,the neurobehavioral scores of each electroacupuncture group were significantly lower than those of the model group(all P<0.05),and the scores showed a significant downward trend as time passed and the frequency of electroacupuncture increased.The neurobehavioral scores of the electroacupuncture group III at 5 d and 10 d were significantly lower than those of the electroacupuncture group I(all P<0.05).HE staining showed that the cells in the cerebral hemorrhage area of the model group were irregularly arranged,a large number of degenerated and necrotic nerve cells wer

关 键 词：脑出血 脑红蛋白 NLRP3信号通路 电针频率 

分 类 号：R-332[医药卫生]