口腔鳞状细胞癌患者循环肿瘤细胞上皮-间充质转化状态的研究  

Detection of Epithelial-mesenchymal Transition Status of Circulating Tumor Cells in Patients with Oral Squamous Cell Carcinoma

作　　者：李杨[1] 李雯[1] 陈茁 李帅泽 乔彬[1] LI Yang;LI Wen;CHEN Zhuo;LI Shuaize;QIAO Bin(Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China)

机构地区：[1]郑州大学第一附属医院口腔颌面外科,河南郑州450052

出　　处：《口腔医学研究》2022年第1期34-38,共5页Journal of Oral Science Research

摘　　要：目的:探讨口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)患者与外周血循环肿瘤细胞(circulating tumor cells,CTCs)的上皮-间充质转化(epithelial-mesenchymal transition,EMT)状态的关系。方法:采用CanPatrolTM CTC-二代捕获技术联合RNA多重原位杂交分析(RNA in situ hybridization,RNA-ISH),检测32例OSCC患者术前1 d外周循环血中的肿瘤细胞。CTCs将根据EMT的相关标志物进行分型鉴定。结果:根据EMT相关标志物的表达情况,将检测出的CTCs分为上皮型CTCs(epithelial CTCs,e-CTCs),混合型CTCs(epithelial-mesenchymal-mixed CTCs,mix-CTCs)及间质型CTCs(mesenchymal CTCs,m-CTCs)。CTCs总数为198,其中e-CTCs为56(28.3%),mix-CTCs为128(64.6%),m-CTCs为14(7.1%)。每位患者的CTCs平均数值为(6.19±6.07),其中包括e-CTCs为(1.75±1.91),mix-CTCs为(4.00±5.77)和m-CTCs为(0.44±0.63)。CTCs总体数值与mix-CTCs数值相关(r^(2)=0.908,P<0.001)。e-CTCs数值与淋巴结有无转移(r^(2)=0.278,P<0.05)和TNM分期(r^(2)=0.278,P<0.05)相关。CTCs总体数值与患者术后发生复发或转移(r^(2)=0.252,P<0.05)相关。结论:mix-CTCs和e-CTCs数值可能对OSCC的进展其重要作用,CTCs可能作为OSCC预测预后的潜在指标。Objective:To investigate the correlation between the status of epithelial-mesenchymal transition(EMT)of circulating tumor cells(CTCs)and oral squamous cell carcinoma(OSCC).Methods:CanPatrol CTC-enrichment and situ hybridizatio(ISH)were used to isolate,identify,and classify CTCs from blood of 32 OSCC patients.Blood was collected from OSCC patients at 1 day before operation.CTCs were identified and characterized according to the EMT markers.Results:CTCs were classified into three subpopulations based on EMT markers:epithelial CTCs(e-CTCs),epithelial-mesenchymal-mixed CTCs(mix-CTC),and mesenchymal CTCs(m-CTCs).Total 198 CTCs were detected in all patients:56(28.3%)CTCs of them were e-CTCs,128(64.6%)were mix-CTCs,and 14(7.1%)were m-CTCs.The average number of CTCs from each patient was 6.19±6.07 which included 1.75±1.91 of e-CTCs,4.00±5.77 of mix-CTCs,and 0.44±0.63 of m---CTCs.The number of total CTCs was correlated with the number of mix-CTCs(r^(2)=0.908,P<0.001).The number of e-CTCs was correlated with lymph node metastasis(r^(2)=0.278,P<0.05)and TNM stage(r^(2)=0.278,P<0.05)in this study.The number of total CTCs was correlated with recurrence or metastasi(r=0.252,P<0.05).Conclusion:Mix-CTCs and e-CTCs might play an important role in the progression of OSCC,and the number of CTCs in OSCC might have the potential to be a predictor of prognosis.

关 键 词：口腔鳞状细胞癌 循环肿瘤细胞 上皮-间充质转化 

分 类 号：R73[医药卫生—肿瘤]