益生菌芦荟发酵凝胶治疗复发性阿弗他溃疡的疗效及对口腔微生物群落的影响  

Efficacy of Probiotic Aloe Vera Fermentation Gel in Treatment of Recurrent Aphthous Ulcer and Its Effect on Oral Microbiome

作　　者：胡晓菁[1] 林璐[1] 黄臻[2] 张停停 刘清 史彦[4] HU Xiaojing;LIN Lu;HUANG Zhen;ZHANG Tingting;LIU Qing;SHI Yan(Department of Oral Medicine, The Affiliated Stomatological Hospital of Nanchang University, The Key Laboratory of Oral Biomedicine of Jiangxi Province, Jiangxi Province Clinical Research Center for Oral Diseases, Nanchang 330006, China;Department of Orthodontics, The Affiliated Stomatological Hospital of Nanchang University, The Key Laboratory of Oral Biomedicine of Jiangxi Province, Jiangxi Province Clinical Research Center for Oral Diseases, Nanchang 330006, China;School of Stomatology, Nanchang University, The Key Laboratory of Oral Biomedicine of Jiangxi Province, Jiangxi Province Clinical Research Center for Oral Diseases, Nanchang 330006, China;Department of Conservative Dentistry and Endodontics, The Affiliated Stomatological Hospital of Nanchang University, The Key Laboratory of Oral Biomedicine of Jiangxi Province, Jiangxi Province Clinical Research Center for Oral Diseases, Nanchang 330006, China)

机构地区：[1]南昌大学附属口腔医院黏膜科,江西省口腔生物医学重点实验室,江西省口腔疾病临床医学研究中心,江西南昌330006 [2]南昌大学附属口腔医院正畸科,江西省口腔生物医学重点实验室,江西省口腔疾病临床医学研究中心,江西南昌330006 [3]南昌大学口腔医学院,江西省口腔生物医学重点实验室,江西省口腔疾病临床医学研究中心,江西南昌330006 [4]南昌大学附属口腔医院牙体牙髓科,江西省口腔生物医学重点实验室,江西省口腔疾病临床医学研究中心,江西南昌330006

出　　处：《口腔医学研究》2022年第1期85-89,共5页Journal of Oral Science Research

基　　金：江西省卫生计生委科技计划—普通科技计划(编号:20175293);江西省教育厅科学技术研究项目-青年项目(编号:60267)。

摘　　要：目的:观察复发性阿弗他溃疡(recurrent aphthous ulcer,RAU)患者使用益生菌芦荟发酵凝胶的临床治疗效果和口腔微生物群落构成改变。方法:随机双盲设计。入选35例RAU患者,患者随机分为试验组和对照组。试验组局部涂抹益生菌芦荟发酵凝胶,对照组局部涂抹壳聚糖凝胶。对比试验组和对照组溃疡愈合天数。未用药前唾液为阳性对照PC组;试验组用药后唾液为AA组和对照组用药后唾液为AC组;另有10名健康人唾液作为阴性对照NC组。高通量测序分析4组唾液(AA、AC、PC、NC)微生物群落之间的变化和相似关联。结果:试验组溃疡愈合天数优于对照组,两组结果具有统计学差异,差异有统计学意义(P<0.05)。门水平:PC组放线菌相对丰度增加,而AA组放线菌相对丰度降低;属水平:PC组嗜血杆菌和奈瑟菌相对丰度下降,放线菌、颗粒菌和胃链球菌相对丰度增加,AA组是正常标准的丰度。结论:益生菌发酵物可以更快的促进溃疡愈合和使口腔微生物更快恢复正常的多样性。Objective:To evaluate the clinical efficacy and oral microbiome composition of patients with recurrent aphthous ulcer treated with probiotic fermentation gel.Methods:A randomized double-blind design was designed to compare the days of ulcer healing between the experimental group and the control group.35 RAU patients were selected and randomly divided into experimental group and control group.Probiotic aloe fermentation gel was applied locally in experimental group and chitosan gel was applied locally in control group.Saliva of RAU patient was collected before using medicine as PC group.The probiotics group(AA)and the control group(AC)were randomly divided into two groups.Saliva of the two groups was collected after treatment.Saliva from 10 healthy persons was used as negative control(NC group).The clinical treatment effect and oral microbiome composition were evaluated.Results:Probiotic fermentation gel had better wound healing effect(P<0.05).At phylum level:the relative abundance of Actinomycetes increased in the PC group,but decreased in the AA group.At genus level:the relative abundance of Haemophilus and Neisseria decreased,but the relative abundance of Actinomycetes,Granulococcus,and Streptococcus Gastric increased in the PC grou;and the relative abundance was normal in the AA group.Conclusion:Probiotic fermentation can accelerate ulcer healing and normalized microbiota disorders of RAU.

关 键 词：复发性阿弗他溃疡 口腔微生态 口腔微生物群落 益生菌 

分 类 号：R781.5[医药卫生—口腔医学]