克氏原螯虾烂尾病病原的分离鉴定及其相关特性分析  

Isolation,Identification and Related Characteristics Analysis of Pathogen Isolated from Red Swamp Crayfish Procambarus clarkii with Tail-Rotted Disease

作　　者：刘张淮 吴霆 王家军 朱春艳 张荧荧 孟庆国 LIU Zhanghuai;WU Ting;WANG Jiajun;ZHU Chunyan;ZHANG Yingying;MENG Qingguo(Baoying Center for Control and Prevention of Aquatic Animal Infectious Disease, Yangzhou 225800, China;Jiangsu Key Laboratory for Aquatic Crustacean Diseases, College of Marine Science and Engineering, Nanjing Normal University, Nanjing 210023, China)

机构地区：[1]宝应县水生动物疫病预防控制中心,江苏扬州225800 [2]南京师范大学海洋科学与工程学院,江苏省水生甲壳动物病害重点实验室,江苏南京210023

出　　处：《水产科学》2022年第1期102-109,共8页Fisheries Science

基　　金：江苏省现代农业(克氏原螯虾)产业技术体系建设项目(JATS[2019]260).

摘　　要：为研究克氏原螯虾烂尾病病原种类及其相关特性,从患病虾尾部病灶分离出2种具有不同菌落形态和色泽的代表菌株,分别编号LW2047-1和LW2047-2,经人工感染试验确定病原菌为菌株LW2047-2。对菌株LW2047-2进行细菌学观察,分析形态特征和生理生化特性,并对16S rRNA和gyrB基因测序分析,最终将其鉴定为弗氏柠檬酸杆菌。检测该菌株定居因子CFA基因和几丁质酶chi基因,分析其黏附性和几丁质分解特性。药物敏感性试验结果表明,菌株LW2047-2对恩诺沙星和硫酸新霉素2种抗菌药物高度敏感,对磺胺类药物均不敏感。The purpose of this experiment was to study the species and related characteristics of pathogen which caused tail-rotted disease of red swamp crayfish Procambarus clarkii.Two representative bacterial strains with different colony morphologies and colors were isolated from infectious location of diseased red swamp crayfish,and known as LW2047-1 and LW2047-2.The pathogen bacteria were confirmed as strain LW2047-2 by artificial infection test.We observed strain LW2047-2 with bacteriological methods and analyzed that by morphological,physiological and biochemical characteristics.Finally,strain LW2047-2 was identified as Citrobacter freundii when 16S rRNA and gyrB gene were sequenced and analyzed.CFA gene and chi gene were detected for analysis of adhesion and chitin decomposition characteristics.Results of antimicrobial sensitivity test showed that strain LW2047-2 was sensitive to enrofloxacin and neomycin sulfate and insensitive to sulfa drugs.

关 键 词：克氏原螯虾 烂尾病 弗氏柠檬酸杆菌 分离鉴定 特性分析 

分 类 号：S948[农业科学—水产养殖]