碘-125粒子调控微小RNA-193b-5p抑制胃癌的增殖和侵袭  

Iodine-125 Seed Inhibits the Proliferation and Invasion of Gastric Cancer Cells by Regulating MicroRNA-193b-5p Expression

作　　者：马振桓 李震[1,2] 周香林[3] 李国剑 杨国凯 万嘉 杜玲娟[1,2] 杨镛 MA Zhenhuan;LI Zhen;ZHOU Xianglin;LI Guojian;YANG Guokai;WAN Jia;DU Lingjuan;YANG Yong(The 3rd Department.of General Surgery,The Affiliated Hospital of Yunnan University,Kunming Yunnan 650021;The 3rd Department of General Surgery,The 4th Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650021;Clinical Medical College,Dali University,Dali Yunnan 671003,China)

机构地区：[1]云南大学附属医院普外三科,云南昆明650021 [2]昆明医科大学第四附属医院普外三科,云南昆明650500 [3]大理大学临床医学院,云南大理671003

出　　处：《昆明医科大学学报》2022年第1期8-13,共6页Journal of Kunming Medical University

基　　金：云南省卫生科技基金资助项目(2017NS137,2018NS0008,2018NS0009);云南省高层次卫生健康技术人才培养专项基金资助项目(H-2017017);云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(2018FE001(-076))。

摘　　要：目的探究碘-125粒子放疗胃癌过程中微小RNA(miR)-193b-5p的作用。方法以胃癌细胞系BGC-823作为对象,建立碘-125粒子体外照射模型,采用小分子RNA测序技术检测miRNA的表达谱。通过实时定量PCR检测碘-125辐照组和非辐照组细胞中miR-193b-5p的表达水平,使用hsa-miR-193b-5p抑制物和相应阴性抑制物转染胃癌细胞系BGC-823建立miR-193b-5p敲减细胞和对照细胞(miR-193b-5p敲减组和对照组),采用噻唑蓝(MTT)检测2组细胞增殖情况,采用Transwell分析2组细胞的侵袭情况。结果碘-125辐照组细胞miR-193b-5p表达水平(0.853±0.180)显著高于非辐照组(0.173±0.045),差异有统计学意义(t=6.371,P<0.05);敲减组细胞miR-193b-5p表达水平(1.264±0.311)明显低于对照组(12.219±2.464),差异有统计学意义(t=-7.640,P<0.05);miR-193b-5p敲减组细胞的增殖能力(0.574±0.382)明显高于对照组细胞(0.424±0.246),差异有统计学意义(t=3.927,P<0.05);miR-193b-5p敲减组细胞侵袭数量(222±23)明显高于对照组细胞侵袭数(40±7),差异有统计学意义(t=-13.293,P<0.05)。结论碘-125放疗胃癌过程中,能通过调控miR-193b-5p的表达抑制胃癌细胞的增殖和侵袭。Objective To investigate the role of microRNA(miRNA,miR)-193 b-5 p in the process of iodine-125 particle radiotherapy for gastric cancer.Methods Gastric cancer cell line BGC-823 was used as the research object and iodine-125 seed in vitro irradiation model was established,and the expression profile of miRNA was detected by small molecule RNA sequencing.The expression of miR-193 b-5 p was analyzed by quantitative real time polymerase chain reaction(RT-qPCR).MiR-193 b-5 p knockdown cell and control cell lines(knockdown group and control group)were established in gastric cancer cell line BGC-823 by hsa-mi R-193 b-5 p inhibitor and NC inhibitor.Cell proliferation was analyzed by methyl thiazolyl tetrazolium(MTT)assay in the two groups.Cell invasion was analyzed by Transwell.Result Compared with the expression level of mi R-193 b-5 p in the nonirradiated group(0.173±0.045),the expression of mi R-193 b-5 p in the iodine-125 irradiated group(0.853±0.180)was significantly increased(t=6.371,P<0.05).Compared with the expression of mi R-193 b-5 p in the control group(12.219±2.464),the expression of mi R-193 b-5 p in the knockdown group(1.264±0.311)was significantly decreased(t=-7.640,P<0.05).Compared with the control group(0.424±0.246),the proliferation of knockdown group(0.574±0.382)was significantly increased(t=3.927,P<0.05).Compared with the control group(40±7),the number of cell invasion in the knockdown group(222+23)was significantly increased(t=-13.293,P<0.05).Conclusion Iodine-125 seed suppresses the proliferation and invasion of gastric cancer cells by regulating the expression of mi R-193 b-5 p in the process of radiotherapy for gastric cancer.

关 键 词：碘-125粒子 胃癌 微小RNA-193b-5p 细胞增殖 细胞侵袭 

分 类 号：R735.2[医药卫生—肿瘤]