miR-138靶向SYT13对胃癌细胞侵袭和迁移的影响及机制研究  

Effects of miR-138 on the invasion and migration of gastric carcinoma cells by targeting SYT13 and its mechanism

作　　者：张雨生 孙博实 迟强[1] ZHANG Yu-sheng;SUN Bo-shi;CHI Qiang(Department of General Surgery,The Second Affiliated Hospital of Harbin Medical University,Harbin 150081,China)

机构地区：[1]哈尔滨医科大学附属第二医院普外科,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2021年第5期495-499,共5页Journal of Harbin Medical University

基　　金：国家自然科学基金资助项目(81802990);黑龙江省卫计委课题(2018345)。

摘　　要：目的探究microRNA-138(miR-138)对胃癌细胞侵袭和迁移能力的影响并探讨其相关机制。方法RT-PCR检测胃癌和癌旁组织中miR-138的表达及miR-138与上皮标志物E-Cadherin及间质标志物Vimentin表达的相关性;使用TargetScan 7.2预测miR-138的可能结合靶点,荧光素酶报告实验、Western blot及RT-PCR检测miR-138对SYT13的调控作用;Transwell侵袭实验和划痕实验检测miR-138调控SYT13对MGC803细胞侵袭迁移能力的影响。结果miR-138在胃癌中表达下调(P<0.001),并与E-Cadherin表达呈正相关(r=0.3022,P<0.05),与Vimentin表达呈负相关(r=0.4762,P<0.05)。miR-138与SYT13存在结合位点,共转染miR-138和SYT13,荧光素酶活性降低(P<0.05),过表达miR-138,SYT13的mRNA(P<0.05)和蛋白(P<0.001)表达被抑制。过表达miR-138抑制MGC803细胞侵袭能力(P<0.001),而同时过表达SYT13,MGC803细胞侵袭能力恢复(P<0.001)。同时miR-138抑制胃癌细胞迁移,而SYT13促进迁移能力。结论miR-138可以通过下调SYT13的表达抑制胃癌MGC803细胞的侵袭和迁移能力。Objective To investigate the role of microRNA-138(miR-138)in the progression of gastric carcinoma and its molecular mechanism.Methods RT-PCR was used to detect the expression of miR-138 in gastric carcinoma and paracancerous tissues.The correlation of miR-138,epithelial marker E-Cadherin and the interstitial marker Vimentin were also evaluated.TargetScan 7.2 was used to predict the possible binding target of miR-138.Luciferase reporter assay,Western blot and RT-PCR were used to verify the regulation of miR-138 on SYT13.Transwell invasion assay and Wound-healing assay were used to detect effect of miR-138 and SYT13 on the invasion and migration ability of MGC803 cells.Results MiR-138 was down-regulated in gastric carcinoma tissues(P<0.001).There was a positive correlation between miR-138 and E-Cadherin expression(r=0.3022,P<0.05),and a negative correlation with Vimentin expression(r=0.4762,P<0.05).MiR-138 and SYT13 have binding sites,co-transfected with miR-138 and SYT13 decreased luciferase activity(P<0.05)and suppressed the overexpressed miR-138,SYT13 mRNA(P<0.05)and protein expression level(P<0.001).In gastric carcinoma cell lines,overexpression of miR-138 inhibited cell invasion(P<0.001),while overexpression of SYT13,gastric carcinoma cell invasion ability recovered(P<0.001).At the same time,miR-138 inhibited the migration of gastric carcinoma cells,while SYT13 promoted migration.Conclusion MiR-138 inhibits the invasion and migration ability of MGC803 cells by down-regulating the expression of SYT13.

关 键 词：miR-138 胃癌 SYT13 侵袭 迁移 

分 类 号：R735.2[医药卫生—肿瘤]