OsTZF9基因过量表达调控水稻多种生长发育表型  

Overexpression of OsTZF9 Gene Regulates Various Phenotypesof Growth and Development in Rice

作　　者：周淑芬[1,2] 刘宝 张迪 ZHOU Shu-fen;LIU Bao;ZHANG Di(Institute of Biological Technology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;Fujian Key Laboratory of Agricultural Genetic Engineering, Fuzhou, Fujian 350003, China)

机构地区：[1]福建省农业科学院生物技术研究所,福建福州350003 [2]福建省农业遗传工程重点实验室,福建福州350003

出　　处：《福建农业科技》2021年第11期1-6,共6页Fujian Agricultural Science and Technology

基　　金：福建省科技计划项目——省属公益类科研院所基本科研专项(2017R1019-3)。

摘　　要：RRTZF基因在植物的生长发育和逆境胁迫中起着重要作用。OsTZF9基因是水稻RRTZF基因家族的一员,为分析OsTZF9基因的生物学功能,构建了由Ubiqutin启动子驱动的植物过表达载体pCXUN-TZF9,通过农杆菌介导法获得了转基因再生植株6个克隆。进一步分析转基因植株后代的表型发现,与野生型日本晴相比,OsTZF9基因过表达植株的株高、分糵数、有效穗数、穗长和千粒重均下降;与转基因阴性分离株相比,OsTZF9基因过表达植株抽穗期推迟。RR-TZF gene plays an important role in plant growth,development and stress responses.OsTZF9 gene is a member of rice RR-TZF gene family.In order to analyze the biological function of OsTZF9 gene,a plant overexpression vector pCXUN-TZF9 driven by Ubiqutin promoter was constructed,and 6 clones of transgenic regenerated plants were obtained by using the agrobacterium-mediated method.Further analysis of the phenotype of the progenies of the transgenic plants showed that plant height,tiller number,effective panicle number,panicle length and 1000-grain weight of the OsTZF9-overexpressed plants were decreased compared with those of the wild-type Nipponbare;compared with the transgenic negative isolated plants,heading date of the OsTZF9-overexpressed plants was delayed.

关 键 词：水稻 OsTZF9基因 过量表达 表型分析 

分 类 号：S511[农业科学—作物学]