哮喘宁颗粒对心理应激哮喘大鼠的治疗作用及抗氧化作用机制  

Therapeutic Effect and Antioxidant Mechanism of Xiaochuanning Granule on Psychological Stress Related Asthma in Rats

作　　者：候丹 黄帅阳 吕明圣 弓雪峰 崔红生 HOU Dan;HUANG Shuai-yang;LYU Ming-sheng;GONG Xue-feng;CUI Hong-sheng(Beijing University of Chinese Medicine Third Affliated Hospital,Beijing 100029,China;Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学第三附属医院,北京100029 [2]北京中医药大学,北京100029

出　　处：《中国实验方剂学杂志》2022年第2期55-61,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金面上项目(82074390)。

摘　　要：目的:观察哮喘宁颗粒对心理应激性哮喘大鼠的治疗作用及抗氧化机制。方法:6周龄雄性SD大鼠随机分为正常组、哮喘组、应激组、应激哮喘组、中药组(哮喘宁颗粒2.48 g·g^(-1)灌胃)、西药组(布地奈德混悬液雾化);第1,8天腹腔注射10%卵蛋白(OVA),氢氧化铝混合液+第15天开始1%OVA溶液雾化激发建立哮喘模型;其中应激组、应激哮喘组、中药组、西药组从第1天起给予束缚刺激,连续刺激28 d,最终建立心理应激性哮喘模型;第15天开始各组连续干预2周。第15,28天进行糖水偏好实验,旷场实验检测;实验结束,检测体质量,血清白细胞介素(IL)-4,IL-5,IL-13含量;肺组织丙二醛(MDA),超氧化物歧化酶(SOD),谷胱甘肽(GSH)水平;肺苏木素-伊红(HE)染色;蛋白免疫印迹法(Western blot)检测肺组织核因子E2相关因子2(Nrf2),血红素氧合酶-1(HO-1)蛋白表达。结果:与应激哮喘组比较,中药组大鼠体质量、糖水消耗率、旷场路程明显升高(P<0.05);血清IL-4,IL-5,IL-13含量明显降低(P<0.05);肺组织SOD,GSH水平明显升高(P<0.05),MDA水平明显降低(P<0.05);HE染色显示中药组支气管黏膜损伤较轻,炎细胞浸润、腺体增生、上皮变性坏死等明显减轻;与应激哮喘组比较,中药组肺组织Nrf2,HO-1蛋白表达明显升高(P<0.05)。结论:哮喘宁颗粒具有调节心理应激性哮喘大鼠心理应激状态作用,同时缓解气道炎症反应、抗氧化,与其上调Nrf2/HO-1蛋白表达相关。Objective:To observe the therapeutic effect and antioxidant mechanism of Xiaochuanning granule on psychological stress-related asthma in rats.Method:The 6-week-old male SD rats were randomly divided into the normal group,asthma group,stress group,stress-related asthma group,western medicine group(atomization of budesonide suspension)and traditional Chinese medicine(TCM)group(Xiaochuanning granule 2.48 g·kg^(-1)).The asthma model was established during 28 days by intraperitoneal injection of 10%ovalbumin(OVA)on the 1st and 8th days and inhaling of vapourized 1%OVA started at the 15th day.Stress group,stress-related asthma group,western medicine group and TCM group were given restraint stimulation during the 28 days to establish the psychological stress-related asthma model.Rats in each group were administered with corresponding drug for 14 days from the 15th day.The sucrose preference test and open field test were performed at the 15th and 28th days.At the end of experiment,the body weight,serum interleukin-4(IL-4),interleukin-5(IL-5)and interleukin-13(IL-13)levels,as well as the levels of malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione(GSH)in lung tissues were detected by assay kits.Hematoxylin-eosin(HE)staining was conducted to observe the pathological changes in lung tissues.Meanwhile,Western blot was used to detect the protein expression of nuclear factor erythroid 2-related factor 2(Nrf2)and hemeoxygenase-1(HO-1)in lung tissues.Result:Compared with the stress-related asthma group,the body weight,sugar water consumption rate and open field distance in the TCM group were significantly increased(P<0.05),and the serum IL-4,IL-5,IL-13 levels were significantly decreased(P<0.05),the levels of SOD and GSH in lung tissues increased significantly(P<0.05),while the level of MDA decreased significantly(P<0.05).HE staining showed that the bronchial mucosal injury,inflammatory cell infiltration,gland hyperplasia,epithelial degeneration and necrosis were significantly ameliorated in the TCM group than in the

关 键 词：哮喘宁颗粒 心理应激哮喘大鼠 心理应激 氧化应激 核因子E_(2)相关因子2/血红素氧合酶1 

分 类 号：R22[医药卫生—中医基础理论] R242[医药卫生—中医学]