运用化学遗传学方法鉴定星形胶质细胞调控抑制性突触形成的关键蛋白  

Chemico-genetic Approach to Identify the Key Proteins Mediating Astrocytic Regulation on Inhibitory Synaptic Formation

作　　者：苏一洵 李晖[1] 易陈菊 SU Yi-xun;LI Hui;YI Chen-ju(Research Center,The Seventh Affiliated Hospital,Sun Yat-sen University,Guangdong 518107,China)

机构地区：[1]中山大学附属第七医院科研中心,广东深圳518107

出　　处：《神经损伤与功能重建》2022年第1期1-3,共3页Neural Injury and Functional Reconstruction

基　　金：国家自然科学基金(No.81971309,No.32170980);广东省自然科学基金(No.2019A1515011333);深圳市科技创新委员会-优秀科技创新人才培养项目(No.RCYX20200714114644167);深圳市科技创新委员会-自然科学基金面上项目(No.JCYJ20210324123212035,No.JCYJ20210324121214039,No.JCYJ20190809161405495)。

摘　　要：星形胶质细胞对神经元突触的形成起着重要作用。但由于缺乏体内研究细胞之间互作蛋白的技术手段,我们对星形胶质细胞与神经元的互作机制了解有限。近来,来自杜克大学的Cagla Eroglu团队和Scott H.Soderling团队在Nature杂志上发表的文章,报道了一种利用在体化学遗传学的Split-TurboID方法,并利用该方法鉴定了星形胶质细胞中与神经元表面互作的蛋白,发现星形胶质细胞NrCAM蛋白可通过与神经元NrCAM互作,负调控星形胶质细胞的体积大小,并在神经元桥尾蛋白介导下促进抑制性突触形成。Objective:Astrocyte plays a crucial role in neuronal synaptic formation.However,our understanding of astrocyte-neuron interaction is hindered by the lack of in vivo cell-cell interaction proteome technique.Recently,a paper published in Nature by Cagla Eroglu and Scott H.Soderling et al.reported a Split-TurboID method[1],utilizing a in vivo chemico-genetic approach to identify the proteome between the astrocyte-neuron cleft.Among these proteins,they found the astrocytic NrCAM negatively regulates astrocyte territory size and neuropil infiltration volume via transmembrane homophilic interaction with neuronal NrCAM.In addition,this interaction promotes inhibitory synaptic formation with the participation of gephyrin.

关 键 词：星形胶质细胞 抑制性突触 Split-TurboID 神经黏附分子 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.02[医药卫生—临床医学]