五种相关光合蛋白基因在马铃薯野生种冷驯化中的表达分析  

Expression Analysis of Five Photosynthesis-related Protein Genes in Cold Acclimation of Wild Potato Varieties

作　　者：吴巧玉 何天久 WU Qiaoyu;HE Tianjiu(Guizhou Academy of Agricultural Sciences Institute of Biotechnology,Guiyang 550006,China)

机构地区：[1]贵州省农业科学院生物技术研究所,贵阳550006

出　　处：《种子》2021年第12期56-60,共5页Seed

基　　金：贵州省科学技术基金项目(黔科合基础[2018]1152);黔农科院国基后补助项目([2021]42)。

摘　　要：以马铃薯野生种S.acaule(W 3,抗冻、具冷驯化能力)和S.cardiophyllum(Cph 12,霜冻敏感、不具冷驯化能力)为研究对象,通过荧光定量PCR,研究五种光合相关蛋白基因在冷驯化期间的转录表达量。结果表明,在冷驯化期间,两种植株叶片净光合速率均呈先降后增趋势;W 3植株叶绿素呈先增后降趋势,Cph 12植株叶片叶绿素变化不明显;叶绿体放氧增强蛋白1、叶绿体锰稳定蛋白-Ⅱ与叶绿体细胞色素f在转录水平先降低,后升高,再降低;叶绿体光系统Ⅱ修复蛋白PSB 27-H 1在转录水平先升高,后降低;叶绿体3-磷酸甘油醛脱氢酶在W 3中先升高,后降低;在Cph 12中先降低,后升高,再降低。前四种光合相关蛋白基因在两种材料中转录水平的表达具有较大的相似性;而叶绿体甘油醛-3-磷酸脱氢酶在W 3进行冷驯化1 h后即开始显著上调表达,第4天表达量显著降低,表明其参与了马铃薯对冷驯化低温环境的应急响应过程。推测光合系统对冷驯化条件的适应可能是马铃薯冷驯化能力实现的必要条件。In this paper, the transcriptional expression levels of five photosynthesis-related proteins genes in wild potato varieties Solanum acaule(W 3,cold-resistant variety) and Solanum cardiophyllum(Cph12, frost-sensitive variety without cold-acclimated capability) during cold acclimation were studied by quantitative PCR. The results showed that the net photosynthetic rate of both plants decreased first and then increased during the cold acclimation period. Chlorophyll in W3 plants increased first and then decreased, while chlorophyll in Cph12 plants did not change significantly. The transcription levels of chloroplast oxygen-enhancing protein 1, chloroplast manganese stable protein-Ⅱ and chloroplast cytochrome f decreased first then increased, and then decreased. The transcription level of PSB 27-H1 in chloroplast photosystem II was firstly increased and then decreased. The transcription level of chloroplast glyceraldehyde 3-phosphate dehydrogenase increased first and then decreased in W3 plants, while first decreased then increased, and then decreased In Cph12 plants. The transcription levels of the first four kinds of photosynthesis-related protein genes in the two materials were similar, while chloroplast glyceraldehyde-3-phosphate dehydrogenase was significantly up-regulated 1 h after cold acclimation of W3 plants, and decreased significantly on day 4, indicating that it was involved in the emergency response of potato to cold acclimation and low temperature. It was speculated that the adaptation of photosynthetic system to cold acclimation may be a necessary condition for the realization of potato cold acclimation ability.

关 键 词：马铃薯 冷驯化 净光合速率 光合蛋白 表达分析 

分 类 号：S532[农业科学—作物学]