苦瓜多糖对小鼠淋巴细胞免疫调节作用的影响  

Effect of Momordica Charantia Polysaccharide on Lymphocyte Immune Regulation in Mice

作　　者：阿尔祖古丽·阿卜力米提 李敬双 金鑫 于洋 ARZUGUL·Ablimiti;LI Jing-shuang;JIN Xin;YU Yang(College of Food Science and Engineering,Jinzhou Medical University,Jinzhou,Liaoning 121001,China;College of Animal Husbandry and Veterinary Medicine,Jinzhou Medical University,Jinzhou,Liaoning 121001,China)

机构地区：[1]锦州医科大学食品科学与工程学院,辽宁锦州121001 [2]锦州医科大学畜牧兽医学院,辽宁锦州121001

出　　处：《粮油食品科技》2022年第1期105-112,共8页Science and Technology of Cereals,Oils and Foods

基　　金：辽宁省自然科学基金(20170540369)。

摘　　要：以苦瓜多糖(MCP)为研究对象,在细胞和分子水平上探讨其对小鼠脾淋巴细胞的免疫调节作用机制。以空白组和左旋咪唑组为对照,小鼠脾淋巴细胞经不同质量浓度(40,80,160,320μg/mL)的MCP体外刺激,检测MCP对小鼠脾淋巴细胞增殖的影响,腹腔巨噬细胞的吞噬活性,淋巴细胞上清液中IL-4、IFN-γ、IL-6和IL-12分泌与mRNA表达水平。分析发现,与空白组相比,随浓度的升高细胞增殖指数倍数增长(P<0.05),MCP能不同程度的促进细胞增殖、不同程度的增强巨噬细胞的吞噬能力(P<0.05);与空白组相较,细胞因子IL-4、IFN-γ、IL-6和IL-12的分泌量最大增幅分别为:42.73%、18%、27.81%、29.42%;IL-4、IFN-γ、IL-6和IL-12的mRNA表达显著上升,最高表达水平分别为空白组的9.18、7.53、9.37、8.95倍,存在显著性差异(P<0.05)。结果表明,MCP能有效提高细胞因子IL-4、IFN-γ、IL-6和IL-12的分泌和mRNA表达水平,促进细胞增殖,增强巨噬细胞的吞噬活性,从而提高机体免疫功能。This study aims to investigate the immunomodulatory effect of Momordica charantia polysaccharide(MCP) on mice spleen lymphocytes and its potential mechanism at the cellular and molecular levels.Compared with the blank group and levamisole group, mice spleen lymphocytes are stimulated by MCP with different concentrations(40, 80, 160, 320 μg/m L) in vitro. The effects of MCP on the proliferation of mice spleen lymphocytes, phagocytic activity of peritoneal macrophages, secretion and m RNA expression of IL-4, IFN-γ, IL-6 and IL-12 in lymphocyte supernatant are examined. The results show that compared with the blank group, the cell proliferation index increases exponentially as the concentration(P<0.05) raises, and MCP promotes cell proliferation in different degrees. MCP enhances phagocytosis of macrophages in different degrees(P<0.05). Compared with the blank group, the secretion of cytokines IL-4, IFN-γ, IL-6 and IL-12 all increase, with the largest increase being 42.73%, 18%, 27.81% and 29.42%, respectively. The m RNA expressions of IL-4, IFN-γ, IL-6 and IL-12 increase significantly, and the highest expression levels are 9.18 times, 7.53 times, 9.37 times and 8.95 times of the blank group, respectively, with significant difference(P<0.05). It is understood that MCP can effectively improve the secretion and m RNA expression of cytokines IL-4, IFN-γ, IL-6 and IL-12, and promote cell proliferation, enhance phagocytic activity of macrophages, thus improving the immune function of the body.

关 键 词：苦瓜多糖 脾淋巴细胞 细胞增值 巨噬细胞 细胞因子 m RNA 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]